{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T06:39:43Z","timestamp":1759991983364},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"2","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1989,10,15]]},"abstract":"<jats:p>Biochemical characterization of the Ca2+-ATPases isolated from human platelet intracellular and plasma membranes is reported. A comparative study of the previously partly described plasma membrane Ca2+-ATPase [Enouf, Bredoux, Bourdeau &amp; Levy-Toledano (1987) J. Biol. Chem. 261, 9293-9297] and the intracellular membrane Ca2+-ATPase obtained simultaneously shows differences in the following parameters: (1) different kinetics of the two enzymes; (2) similar apparent affinity towards Ca2+ (10(-7) M), though the intracellular membrane enzyme was inhibited at Ca2+ concentrations above 10(-6) M; (3) different pH dependence with an activity maximum at pH 7 for the intracellular membrane Ca2+-ATPase and no detectable pH maximum for the plasma membrane Ca2+-ATPase; (4) a 10-fold difference in the ATP requirement of the two Ca2+-ATPases; (5) different patterns of inhibition by vanadate. Finally, the possible regulation of the Ca2+-ATPases was examined by studying the effect of chlorpromazine on the two Ca2+-ATPase activities, with only the plasma membrane enzyme being inhibited. It is concluded that the two platelet Ca2+ transport systems show biochemical differences in spite of the previously shown similarity in the molecular masses of their Ca2+-ATPases, thus conferring a definite specificity to the platelet system.<\/jats:p>","DOI":"10.1042\/bj2630547","type":"journal-article","created":{"date-parts":[[2015,8,10]],"date-time":"2015-08-10T21:14:21Z","timestamp":1439241261000},"page":"547-552","update-policy":"http:\/\/dx.doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["Further characterization of the plasma membrane- and intracellular membrane-associated platelet Ca2+ transport systems"],"prefix":"10.1042","volume":"263","author":[{"given":"J","family":"Enouf","sequence":"first","affiliation":[{"name":"*Unit\u00e9 INSERM No. 150, Unit\u00e9 Associ\u00e9e CNRS No. 334, H\u00f4pital Lariboisi\u00e8re, 6 rue Guy Patin, 75475 Paris Cedex 10, France."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R","family":"Bredoux","sequence":"additional","affiliation":[{"name":"*Unit\u00e9 INSERM No. 150, Unit\u00e9 Associ\u00e9e CNRS No. 334, H\u00f4pital Lariboisi\u00e8re, 6 rue Guy Patin, 75475 Paris Cedex 10, France."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N","family":"Bourdeau","sequence":"additional","affiliation":[{"name":"*Unit\u00e9 INSERM No. 150, Unit\u00e9 Associ\u00e9e CNRS No. 334, H\u00f4pital Lariboisi\u00e8re, 6 rue Guy Patin, 75475 Paris Cedex 10, France."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B","family":"Sarkadi","sequence":"additional","affiliation":[{"name":"*Unit\u00e9 INSERM No. 150, Unit\u00e9 Associ\u00e9e CNRS No. 334, H\u00f4pital Lariboisi\u00e8re, 6 rue Guy Patin, 75475 Paris Cedex 10, France."},{"name":"National Institute of Haematology and Blood Transfusion, Budapest, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S","family":"Levy-Toledano","sequence":"additional","affiliation":[{"name":"*Unit\u00e9 INSERM No. 150, Unit\u00e9 Associ\u00e9e CNRS No. 334, H\u00f4pital Lariboisi\u00e8re, 6 rue Guy Patin, 75475 Paris Cedex 10, France."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","container-title":["Biochemical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/263\/2\/547\/597916\/bj2630547.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/263\/2\/547\/597916\/bj2630547.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,25]],"date-time":"2021-11-25T18:10:19Z","timestamp":1637863819000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/biochemj\/article\/263\/2\/547\/24638\/Further-characterization-of-the-plasma-membrane"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1989,10,15]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1989,10,15]]}},"URL":"https:\/\/doi.org\/10.1042\/bj2630547","relation":{},"ISSN":["0264-6021","1470-8728"],"issn-type":[{"value":"0264-6021","type":"print"},{"value":"1470-8728","type":"electronic"}],"subject":[],"published":{"date-parts":[[1989,10,15]]}}}