{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T15:59:05Z","timestamp":1762271945420},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"1","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1990,1,1]]},"abstract":"<jats:p>Repeated administration of the beta-adrenergic agonist isoprenaline (isoproterenol, IPR), which produces hypertrophic\/hyperplastic enlargements of rat submandibular and parotid glands, induces synthesis of a secretory protein shown to be a cysteine proteinase inhibitor, rat cystatin S. In the current study, Northern blot and hybridizations in situ were carried out to establish the developmental and beta-adrenergic regulation of the expression of the cystatin S gene. Cystatin S mRNA was not detected in submandibular glands of 20-day-old fetuses, nor in the glands of newborn or 10-day-old rats. However, steady-state levels of cystatin S mRNA increased between 21 and 28 days, reaching a conspicuously high concentration at 28 days; cystatin S mRNA then declined rapidly to a barely detectable level in glands of 32-day-old rats. IPR administration for 4 days induced high levels of cystatin S mRNA in submandibular glands of developing and adult rats. In both prepubertal and mature animals, induction of cystatin S mRNA in submandibular glands was more pronounced in female than in male animals. Hybridizations in situ revealed cystatin S mRNA only in acinar but not in duct cells of the submandibular gland. Developmentally, expression of the cystatin S gene coincided with acinar cell differentiation. These data suggest a complex neural, hormonal and developmental regulation of salivary cystatin genes.<\/jats:p>","DOI":"10.1042\/bj2650115","type":"journal-article","created":{"date-parts":[[2015,8,10]],"date-time":"2015-08-10T21:14:47Z","timestamp":1439241287000},"page":"115-120","update-policy":"http:\/\/dx.doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":26,"title":["Expression and induction by <i>\u03b2<\/i>-adrenergic agonists of the cystatin S gene in submandibular glands of developing rats"],"prefix":"10.1042","volume":"265","author":[{"given":"P A","family":"Shaw","sequence":"first","affiliation":[{"name":"Department of Cell Biology\/Anatomy, Mount Sinai School of Medicine, 1 Gustave L. Levy Place, New York, NY 10029, U.S.A."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T","family":"Barka","sequence":"additional","affiliation":[{"name":"Department of Cell Biology\/Anatomy, Mount Sinai School of Medicine, 1 Gustave L. Levy Place, New York, NY 10029, U.S.A."},{"name":"Department of Pathology, Mount Sinai School of Medicine, 1 Gustave L. Levy Place, New York, NY 10029, U.S.A."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A","family":"Woodin","sequence":"additional","affiliation":[{"name":"Department of Dartmouth Medical School, Hanover, NH 03756, U.S.A."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B S","family":"Schacter","sequence":"additional","affiliation":[{"name":"Department of Cell Biology\/Anatomy, Mount Sinai School of Medicine, 1 Gustave L. Levy Place, New York, NY 10029, U.S.A."},{"name":"Department of Obstetrics and Gynecology, Mount Sinai School of Medicine, 1 Gustave L. Levy Place, New York, NY 10029, U.S.A."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J L","family":"Cox","sequence":"additional","affiliation":[{"name":"Department of Cell Biology\/Anatomy, Mount Sinai School of Medicine, 1 Gustave L. Levy Place, New York, NY 10029, U.S.A."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","container-title":["Biochemical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/265\/1\/115\/598197\/bj2650115.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/265\/1\/115\/598197\/bj2650115.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,25]],"date-time":"2021-11-25T18:12:20Z","timestamp":1637863940000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/biochemj\/article\/265\/1\/115\/24794\/Expression-and-induction-by-adrenergic-agonists-of"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1990,1,1]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1990,1,1]]}},"URL":"https:\/\/doi.org\/10.1042\/bj2650115","relation":{},"ISSN":["0264-6021","1470-8728"],"issn-type":[{"value":"0264-6021","type":"print"},{"value":"1470-8728","type":"electronic"}],"subject":[],"published":{"date-parts":[[1990,1,1]]}}}