{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,9,16]],"date-time":"2023-09-16T22:30:15Z","timestamp":1694903415260},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"1","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1990,8,15]]},"abstract":"<jats:p>Chromaffin-granule membranes were separated into insoluble and soluble fractions after extraction with lithium di-iodosalicylate (LDIS). These fractions were characterized by one- and two-dimensional gel electrophoresis, and glycoproteins were detected after electroblotting with peroxidase-labelled concanavalin A and wheat-germ agglutinin (WGA). The LDIS-insoluble fraction contained components identified as glycoproteins III, H, J and K (carboxypeptidase H). Microsequence analysis indicated that component J is an N-terminally extended form of glycoprotein K. A major glycoprotein, GpII (Mr 80,000-100,000), present in the LDIS-soluble fraction was purified by affinity chromatography on WGA-Sepharose. This was characterized by one- and two-dimensional gel electrophoresis with Coomassie Blue staining, by amino acid analysis and automated N-terminal sequence analysis. Extraction of chromaffin-granule membranes with LDIS is a simple and rapid procedure that facilitates studies concerned with the structure and function of membrane glycoproteins from these and other secretory granules.<\/jats:p>","DOI":"10.1042\/bj2700057","type":"journal-article","created":{"date-parts":[[2015,8,10]],"date-time":"2015-08-10T21:17:39Z","timestamp":1439241459000},"page":"57-61","update-policy":"http:\/\/dx.doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Identification and characterization of glycoproteins after extraction of bovine chromaffin-granule membranes with lithium di-iodosalicylate. Purification of glycoprotein II from the soluble fraction"],"prefix":"10.1042","volume":"270","author":[{"given":"D L","family":"Christie","sequence":"first","affiliation":[{"name":"Department of Biochemistry, University of Auckland, Private Bag, Auckland, New Zealand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D J","family":"Palmer","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, University of Auckland, Private Bag, Auckland, New Zealand"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","container-title":["Biochemical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/270\/1\/57\/602466\/bj2700057.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/270\/1\/57\/602466\/bj2700057.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,25]],"date-time":"2021-11-25T18:37:43Z","timestamp":1637865463000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/biochemj\/article\/270\/1\/57\/26301\/Identification-and-characterization-of"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1990,8,15]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1990,8,15]]}},"URL":"https:\/\/doi.org\/10.1042\/bj2700057","relation":{},"ISSN":["0264-6021","1470-8728"],"issn-type":[{"value":"0264-6021","type":"print"},{"value":"1470-8728","type":"electronic"}],"subject":[],"published":{"date-parts":[[1990,8,15]]}}}