{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T13:23:01Z","timestamp":1761744181924},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"3","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1990,11,1]]},"abstract":"<jats:p>The gamma-subunit of retinal rod-outer-segment phosphodiesterase (PDE-gamma) is a multifunctional protein which interacts directly with both of the catalytic subunits of PDE (PDE alpha\/beta) and the alpha-subunit of the retinal G (guanine-nucleotide-binding)-protein transducin alpha (T alpha). We have previously reported that the PDE gamma binds to T alpha at residue nos. 24-45 [Morrison. Rider &amp; Takemoto (1987) FEBS Lett. 222, 266-270]. In vitro this results in inhibition of T alpha GTP\/GDP exchange [Morrison, Cunnick, Oppert &amp; Takemoto (1989) J. Biol. Chem. 264, 11671-11681]. We now report that the inhibitory region of PDE gamma for PDE alpha\/beta occurs at PDE gamma residues 54-87. This binding results in inhibition of either trypsin-solubilized or membrane-bound PDE alpha\/beta. PDE gamma which has been treated with carboxypeptidase Y, removing the C-terminus, does not inhibit PDE alpha\/beta, but does inhibit T alpha GTP\/GDP exchange. Inhibition by PDE gamma can be removed by T alpha-guanosine 5\u2032-[gamma-thio]triphosphate (GTP[S]) addition to membranes. This results in a displacement of PDE gamma, but not in removal of this subunit from the membrane [Whalen, Bitensky &amp; Takemoto (1990) Biochem. J. 265, 655-658]. These results suggest that low levels of T alpha-GTP[S] can result in displacement of PDE gamma from the membrane in vitro as a GTP[S]-T alpha-PDE gamma complex. Further activation by high levels of T alpha-GTP[S] occurs by displacement of PDE gamma from its inhibitory site on PDE alpha\/beta, but not in removal from the membrane.<\/jats:p>","DOI":"10.1042\/bj2710721","type":"journal-article","created":{"date-parts":[[2015,8,10]],"date-time":"2015-08-10T21:18:17Z","timestamp":1439241497000},"page":"721-727","update-policy":"http:\/\/dx.doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Binding of the <i>\u03b3<\/i>-subunit of retinal rod-outer-segment phosphodiesterase with both transducin and the catalytic subunits of phosphodiesterase"],"prefix":"10.1042","volume":"271","author":[{"given":"J M","family":"Cunnick","sequence":"first","affiliation":[{"name":"Department of Biochemistry, Kansas State University, Willard Hall, Manhattan, KS 66506, U.S.A."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D","family":"Hurt","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Kansas State University, Willard Hall, Manhattan, KS 66506, U.S.A."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B","family":"Oppert","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Kansas State University, Willard Hall, Manhattan, KS 66506, U.S.A."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K","family":"Sakamoto","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Kansas State University, Willard Hall, Manhattan, KS 66506, U.S.A."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D J","family":"Takemoto","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Kansas State University, Willard Hall, Manhattan, KS 66506, U.S.A."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","container-title":["Biochemical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/271\/3\/721\/602124\/bj2710721.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/271\/3\/721\/602124\/bj2710721.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,25]],"date-time":"2021-11-25T18:44:55Z","timestamp":1637865895000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/biochemj\/article\/271\/3\/721\/26531\/Binding-of-the-subunit-of-retinal-rod-outer"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1990,11,1]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1990,11,1]]}},"URL":"https:\/\/doi.org\/10.1042\/bj2710721","relation":{},"ISSN":["0264-6021","1470-8728"],"issn-type":[{"value":"0264-6021","type":"print"},{"value":"1470-8728","type":"electronic"}],"subject":[],"published":{"date-parts":[[1990,11,1]]}}}