{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T15:30:04Z","timestamp":1790695804369,"version":"4.1.0"},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"3","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1990,11,1]]},"abstract":"<jats:p>GCDFP(gross-cystic-disease-fluid protein)-24, a progesterone-binding protein present in large amounts in cyst fluid from human breast gross cystic disease, was purified in a one-step procedure by size-exclusion h.p.l.c. Peptide fragments obtained by trypsin digestion of the intact protein were purified by reverse-phase h.p.l.c. and analysed for their amino acid composition and subjected to automated Edman degradation. A search of the National Biomedical Research Foundation Data Bank revealed that all the sequenced tryptic peptides from protein GCDFP-24 matched perfectly with regions present in the amino acid sequence determined for human apolipoprotein D. Additional data on N-terminal sequence of the unblocked proteins, carbohydrate-attachment sites, amino acid composition and molecular-mass estimations supported the identity between both molecules. On the basis of this identity a possible role of apolipoprotein D in progesterone transport is proposed.<\/jats:p>","DOI":"10.1042\/bj2710803","type":"journal-article","created":{"date-parts":[[2015,8,10]],"date-time":"2015-08-10T21:18:17Z","timestamp":1439241497000},"page":"803-807","update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":91,"title":["Apolipoprotein D is the major protein component in cyst fluid from women with human breast gross cystic disease"],"prefix":"10.1042","volume":"271","author":[{"given":"M","family":"Balb\u00edn","sequence":"first","affiliation":[{"name":"Departamento de Biolog\u00eda Funcional, Facultad de Medicina, Universidad de Oviedo, 33006, Oviedo, Spain."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J M","family":"Freije","sequence":"additional","affiliation":[{"name":"Departamento de Biolog\u00eda Funcional, Facultad de Medicina, Universidad de Oviedo, 33006, Oviedo, Spain."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A","family":"Fueyo","sequence":"additional","affiliation":[{"name":"Departamento de Biolog\u00eda Funcional, Facultad de Medicina, Universidad de Oviedo, 33006, Oviedo, Spain."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L M","family":"S\u00e1nchez","sequence":"additional","affiliation":[{"name":"Departamento de Biolog\u00eda Funcional, Facultad de Medicina, Universidad de Oviedo, 33006, Oviedo, Spain."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C","family":"L\u00f3pez-Ot\u00edn","sequence":"additional","affiliation":[{"name":"Departamento de Biolog\u00eda Funcional, Facultad de Medicina, Universidad de Oviedo, 33006, Oviedo, Spain."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"288","container-title":["Biochemical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/271\/3\/803\/601949\/bj2710803.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/271\/3\/803\/601949\/bj2710803.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,25]],"date-time":"2021-11-25T18:42:40Z","timestamp":1637865760000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/biochemj\/article\/271\/3\/803\/26437\/Apolipoprotein-D-is-the-major-protein-component-in"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1990,11,1]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1990,11,1]]}},"URL":"https:\/\/doi.org\/10.1042\/bj2710803","relation":{},"ISSN":["0264-6021","1470-8728"],"issn-type":[{"value":"0264-6021","type":"print"},{"value":"1470-8728","type":"electronic"}],"subject":[],"published":{"date-parts":[[1990,11,1]]}}}