{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T16:42:35Z","timestamp":1762101755681},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"2","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1990,12,1]]},"abstract":"<jats:p>The effects of arachidonic acid and thrombin on calcium movements have been studied in fura-2-loaded platelets by a procedure which allows simultaneous monitoring of the uptake of manganese, a calcium surrogate for Ca2+ channels, and the release of Ca2+ from intracellular stores. Arachidonic acid induced both Ca2+ (Mn2+) entry through the plasma membrane and Ca2+ release from the intracellular stores. The release of Ca2+ was prevented by cyclo-oxygenase inhibitors and mimicked by the prostaglandin H2\/thromboxane A2 receptor agonist U46619. Ca2+ (Mn2+) entry required higher concentrations of arachidonic acid and was not prevented by either cyclo-oxygenase or lipoxygenase inhibitors. Several polyunsaturated fatty acids reproduced the effect of arachidonic acid on Ca2+ (Mn2+) entry, but higher concentrations were required. The effects of maximal concentrations of arachidonic acid and thrombin on the uptake of Mn2+ were not additive. Both agonists induced the entry of Ca2+, Mn2+, Co2+ and Ba2+, but not Ni2+, which, in addition, blocked the entry of the other divalent cations. However, arachidonic acid, but not thrombin, increased a Ni2(+)-sensitive permeability to Mg2+. The effect of thrombin but not that of arachidonic acid was prevented either by pretreatment with phorbol ester or by an increase in cyclic-AMP levels. Arachidonic acid also accelerated the uptake of Mn2+ by human neutrophils, rat thymocytes and Ehrlich ascites-tumour cells.<\/jats:p>","DOI":"10.1042\/bj2720435","type":"journal-article","created":{"date-parts":[[2015,8,10]],"date-time":"2015-08-10T21:18:57Z","timestamp":1439241537000},"page":"435-443","update-policy":"http:\/\/dx.doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":34,"title":["Arachidonic acid-induced calcium influx in human platelets. Comparison with the effect of thrombin"],"prefix":"10.1042","volume":"272","author":[{"given":"M T","family":"Alonso","sequence":"first","affiliation":[{"name":"Departamento de Fisiolog\u00eda y Bioqu\u00edmica, Facultad de Medicina, Universidad de Valladolid, 47005-Valladolid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A","family":"Sanchez","sequence":"additional","affiliation":[{"name":"Departamento de Fisiolog\u00eda y Bioqu\u00edmica, Facultad de Medicina, Universidad de Valladolid, 47005-Valladolid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J","family":"Garcia-Sancho","sequence":"additional","affiliation":[{"name":"Departamento de Fisiolog\u00eda y Bioqu\u00edmica, Facultad de Medicina, Universidad de Valladolid, 47005-Valladolid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","container-title":["Biochemical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/272\/2\/435\/601114\/bj2720435.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/272\/2\/435\/601114\/bj2720435.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,25]],"date-time":"2021-11-25T18:24:56Z","timestamp":1637864696000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/biochemj\/article\/272\/2\/435\/25869\/Arachidonic-acid-induced-calcium-influx-in-human"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1990,12,1]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1990,12,1]]}},"URL":"https:\/\/doi.org\/10.1042\/bj2720435","relation":{},"ISSN":["0264-6021","1470-8728"],"issn-type":[{"value":"0264-6021","type":"print"},{"value":"1470-8728","type":"electronic"}],"subject":[],"published":{"date-parts":[[1990,12,1]]}}}