{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T02:53:04Z","timestamp":1792119184140,"version":"4.4.0"},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"2","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1991,1,15]]},"abstract":"<jats:p>1-Methyl-4-phenylpyridinium (MPP+), the neurotoxic bioactivation product of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP), interrupts mitochondrial electron transfer at the NADH dehydrogenase-ubiquinone junction, as do the respiratory chain inhibitors rotenone, piericidin A and barbiturates. Proof that these classical respiratory chain inhibitors and MPP+ react at the same site in the complex NADH dehydrogenase molecule has been difficult to obtain because none of these compounds bind covalently to the target. The 4\u2032-alkyl derivatives of MPP+ inhibit NADH oxidation in submitochondrial particles at much lower concentrations than does MPP+ itself, but still dissociate on washing the membrane preparations, with consequent re-activation of the enzyme. The MPP+ analogues with short alkyl chains prevent the binding of 14C-labelled piericidin A to the membrane and thus must act at the same site, but analogues with alkyl chains longer than heptyl do not prevent binding of [14C]piericidin.<\/jats:p>","DOI":"10.1042\/bj2730481","type":"journal-article","created":{"date-parts":[[2015,8,10]],"date-time":"2015-08-10T21:19:43Z","timestamp":1439241583000},"page":"481-484","update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":39,"title":["Evidence that the inhibition sites of the neurotoxic amine 1-methyl-4-phenylpyridinium (MPP+) and of the respiratory chain inhibitor piericidin A are the same"],"prefix":"10.1042","volume":"273","author":[{"given":"R R","family":"Ramsay","sequence":"first","affiliation":[{"name":"Department of Biochemistry and Biophysics, University of California, San Francisco, CA 94143"},{"name":"Molecular Biology Division, Department of Veterans Affairs Medical Center, San Francisco, CA 94121, U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M J","family":"Krueger","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Biophysics, University of California, San Francisco, CA 94143"},{"name":"Molecular Biology Division, Department of Veterans Affairs Medical Center, San Francisco, CA 94121, U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S K","family":"Youngster","sequence":"additional","affiliation":[{"name":"Department of Neurology, University of Medicine and Dentistry of New Jersey-Robert Wood Johnson Medical School, Piscataway, NJ 08854, U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T P","family":"Singer","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Biophysics, University of California, San Francisco, CA 94143"},{"name":"Molecular Biology Division, Department of Veterans Affairs Medical Center, San Francisco, CA 94121, U.S.A."},{"name":"Division of Toxicology, University of California, San Francisco, CA 94143"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"288","container-title":["Biochemical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/273\/2\/481\/602106\/bj2730481.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/273\/2\/481\/602106\/bj2730481.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,23]],"date-time":"2021-11-23T15:05:41Z","timestamp":1637679941000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/biochemj\/article\/273\/2\/481\/26588\/Evidence-that-the-inhibition-sites-of-the"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1991,1,15]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1991,1,15]]}},"URL":"https:\/\/doi.org\/10.1042\/bj2730481","relation":{},"ISSN":["0264-6021","1470-8728"],"issn-type":[{"value":"0264-6021","type":"print"},{"value":"1470-8728","type":"electronic"}],"subject":[],"published":{"date-parts":[[1991,1,15]]}}}