{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,13]],"date-time":"2026-02-13T08:49:04Z","timestamp":1770972544192,"version":"3.50.1"},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"1","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1991,2,15]]},"abstract":"<jats:p>We have studied the mechanism of the regulation of plasma membrane Ca2+ permeability by the degree of filling of the intracellular Ca2+ stores. Using Mn2+ as a Ca2+ surrogate for plasma membrane Ca2+ channels, we found that Mn2+ uptake by rat thymocytes is inversely related to the degree of filling of the intracellular Ca2+ stores. This store-dependent plasma membrane permeability is inhibited by oxygen scavenging, CO, imidazole antimycotics and other cytochrome P-450 inhibitors. The pattern of inhibition is similar to that reported previously for the inhibition of microsomal cytochrome P-450-mediated aryl hydrocarbon hydroxylase activity of lymphocytes. Several calmodulin antagonists, both phenothiazinic (trifluoperazine, fluphenazine and chlorpromazine) and dibenzodiazepinic (clozapine), accelerate Mn2+ uptake by cells with Ca2(+)-filled stores, and this effect is prevented by imidazole antimycotics. Our results suggest that cytochrome P-450 may be the link between the stores and the plasma membrane Ca2+ pathway. We propose a model in which this cytochrome, sited at the stores, stimulates plasma membrane Ca2+ influx. This stimulatory effect is, in turn, prevented by the presence of Ca2+ inside the stores, possibly via a calmodulin-dependent mechanism.<\/jats:p>","DOI":"10.1042\/bj2740193","type":"journal-article","created":{"date-parts":[[2015,8,10]],"date-time":"2015-08-10T21:20:16Z","timestamp":1439241616000},"page":"193-197","update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":124,"title":["Cytochrome <i>P<\/i>-450 may link intracellular Ca2+ stores with plasma membrane Ca2+ influx"],"prefix":"10.1042","volume":"274","author":[{"given":"J","family":"Alvarez","sequence":"first","affiliation":[{"name":"Departamento de Bioqu\u00edmica, Biolog\u00eda Molecular y Fisiolog\u00eda, Facultad de Medicina, Universidad de Valladolid, 47005-Valladolid, Spain."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M","family":"Montero","sequence":"additional","affiliation":[{"name":"Departamento de Bioqu\u00edmica, Biolog\u00eda Molecular y Fisiolog\u00eda, Facultad de Medicina, Universidad de Valladolid, 47005-Valladolid, Spain."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J","family":"Garc\u00eda-Sancho","sequence":"additional","affiliation":[{"name":"Departamento de Bioqu\u00edmica, Biolog\u00eda Molecular y Fisiolog\u00eda, Facultad de Medicina, Universidad de Valladolid, 47005-Valladolid, Spain."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","container-title":["Biochemical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/274\/1\/193\/602517\/bj2740193.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/274\/1\/193\/602517\/bj2740193.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,23]],"date-time":"2021-11-23T15:03:35Z","timestamp":1637679815000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/biochemj\/article\/274\/1\/193\/26386\/Cytochrome-P-450-may-link-intracellular-Ca2-stores"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1991,2,15]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1991,2,15]]}},"URL":"https:\/\/doi.org\/10.1042\/bj2740193","relation":{},"ISSN":["0264-6021","1470-8728"],"issn-type":[{"value":"0264-6021","type":"print"},{"value":"1470-8728","type":"electronic"}],"subject":[],"published":{"date-parts":[[1991,2,15]]}}}