{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,3]],"date-time":"2025-11-03T09:12:42Z","timestamp":1762161162927},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"2","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1991,9,1]]},"abstract":"<jats:p>The intraperitoneal injection of zymosan in the rabbit results in the generation of an inflammatory exudate containing oedema-forming and chemoattractant activities. Previous studies demonstrated the early appearance of the complement fragment C5a, followed by the generation of two mediators related to the cytokine interleukin-8 that were separable by cation-exchange h.p.l.c. N-Terminal amino acid sequencing identified one of these mediators as rabbit interleukin-8. This paper describes the purification of the second cytokine by cation-exchange, gel-filtration and reversed-phase h.p.l.c. The purified material had both oedema-forming and chemoattractant activity when assayed in rabbit skin in vivo. On SDS\/PAGE a single 6-8 kDa band was observed and N-terminal amino acid sequencing of the reduced and alkylated protein positively identified 36 amino acids. This sequence revealed the rabbit homologue of melanoma-growth-stimulatory activity. The identification of these two cytokines in vivo will provide an opportunity to investigate the importance of their co-release in the inflammatory process.<\/jats:p>","DOI":"10.1042\/bj2780493","type":"journal-article","created":{"date-parts":[[2015,8,13]],"date-time":"2015-08-13T21:39:42Z","timestamp":1439501982000},"page":"493-497","update-policy":"http:\/\/dx.doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":25,"title":["Identification of a second neutrophil-chemoattractant cytokine generated during an inflammatory reaction in the rabbit peritoneal cavity <i>in vivo<\/i>. Purification, partial amino acid sequence and structural relationship to melanoma-growth-stimulatory activity"],"prefix":"10.1042","volume":"278","author":[{"given":"P J","family":"Jose","sequence":"first","affiliation":[{"name":"Department of Applied Pharmacology, National Heart and Lung Institute, Dovehouse Street, London SW3 6LY."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P D","family":"Collins","sequence":"additional","affiliation":[{"name":"Department of Applied Pharmacology, National Heart and Lung Institute, Dovehouse Street, London SW3 6LY."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J A","family":"Perkins","sequence":"additional","affiliation":[{"name":"Department of Applied Pharmacology, National Heart and Lung Institute, Dovehouse Street, London SW3 6LY."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B C","family":"Beaubien","sequence":"additional","affiliation":[{"name":"Department of Applied Pharmacology, National Heart and Lung Institute, Dovehouse Street, London SW3 6LY."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N F","family":"Totty","sequence":"additional","affiliation":[{"name":"Ludwig Institute for Cancer Research, Riding House Street, London WIP 8BT, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M D","family":"Waterfield","sequence":"additional","affiliation":[{"name":"Ludwig Institute for Cancer Research, Riding House Street, London WIP 8BT, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J","family":"Hsuan","sequence":"additional","affiliation":[{"name":"Ludwig Institute for Cancer Research, Riding House Street, London WIP 8BT, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T J","family":"Williams","sequence":"additional","affiliation":[{"name":"Department of Applied Pharmacology, National Heart and Lung Institute, Dovehouse Street, London SW3 6LY."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","container-title":["Biochemical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/278\/2\/493\/603847\/bj2780493.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/278\/2\/493\/603847\/bj2780493.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,23]],"date-time":"2021-11-23T15:30:34Z","timestamp":1637681434000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/biochemj\/article\/278\/2\/493\/27273\/Identification-of-a-second-neutrophil"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1991,9,1]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1991,9,1]]}},"URL":"https:\/\/doi.org\/10.1042\/bj2780493","relation":{},"ISSN":["0264-6021","1470-8728"],"issn-type":[{"value":"0264-6021","type":"print"},{"value":"1470-8728","type":"electronic"}],"subject":[],"published":{"date-parts":[[1991,9,1]]}}}