{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,13]],"date-time":"2026-03-13T18:53:40Z","timestamp":1773428020708,"version":"3.50.1"},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"3","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1991,9,15]]},"abstract":"<jats:p>In order to investigate the regulation of canalicular organic-anion transport, we used a hepatocyte transport assay in which canalicular secretion of a model organic anion, dinitrophenyl-glutathione (GS-DNP), was measured in the presence of stimulators and inhibitors of the Ca2+\/protein kinase C (PKC) second-messenger system and of the cyclic AMP (cAMP) second-messenger system. Vasopressin (24 nM) and the phorbol ester phorbol 12-myristate 13-acetate (1 microgram\/ml), both stimulators of PKC, stimulated GS-DNP efflux by 65 +\/- 36% and 55 +\/- 28% respectively, whereas staurosporine (10 microM), an inhibitor of PKC, inhibited efflux by 53 +\/- 13%. Glucagon and forskolin, both stimulators of the cAMP second-messenger system, as well as the cAMP analogue dibutyryl cAMP and the phosphodiesterase inhibitor 3-isobutyl-1-methylxanthine, did not significantly influence the GS-DNP efflux. It can be concluded that canalicular organic-anion transport in hepatocytes is either directly or indirectly regulated by PKC.<\/jats:p>","DOI":"10.1042\/bj2780637","type":"journal-article","created":{"date-parts":[[2015,8,13]],"date-time":"2015-08-13T21:39:42Z","timestamp":1439501982000},"page":"637-641","update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":35,"title":["Hepatocanalicular organic-anion transport is regulated by protein kinase C"],"prefix":"10.1042","volume":"278","author":[{"given":"H","family":"Roelofsen","sequence":"first","affiliation":[{"name":"Division of Gastrointestinal and Liver Diseases, Academic Medical Centre FO-116, Meibergdreef 9, 1105 AZ Amsterdam, The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R","family":"Ottenhoff","sequence":"additional","affiliation":[{"name":"Division of Gastrointestinal and Liver Diseases, Academic Medical Centre FO-116, Meibergdreef 9, 1105 AZ Amsterdam, The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R P J","family":"Oude Elferink","sequence":"additional","affiliation":[{"name":"Division of Gastrointestinal and Liver Diseases, Academic Medical Centre FO-116, Meibergdreef 9, 1105 AZ Amsterdam, The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P L M","family":"Jansen","sequence":"additional","affiliation":[{"name":"Division of Gastrointestinal and Liver Diseases, Academic Medical Centre FO-116, Meibergdreef 9, 1105 AZ Amsterdam, The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","container-title":["Biochemical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/278\/3\/637\/604110\/bj2780637.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/278\/3\/637\/604110\/bj2780637.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,23]],"date-time":"2021-11-23T15:31:20Z","timestamp":1637681480000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/biochemj\/article\/278\/3\/637\/27297\/Hepatocanalicular-organic-anion-transport-is"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1991,9,15]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1991,9,15]]}},"URL":"https:\/\/doi.org\/10.1042\/bj2780637","relation":{},"ISSN":["0264-6021","1470-8728"],"issn-type":[{"value":"0264-6021","type":"print"},{"value":"1470-8728","type":"electronic"}],"subject":[],"published":{"date-parts":[[1991,9,15]]}}}