{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,13]],"date-time":"2026-02-13T14:53:45Z","timestamp":1770994425462,"version":"3.50.1"},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"2","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1992,1,15]]},"abstract":"<jats:p>Inositol 1,4,5-trisphosphate (InsP3), a product of G-protein-mediated receptor activation of phosphoinositide turnover, plays the role of a second messenger when olfactory neurons are stimulated with certain olfactory stimuli. In this paper we examine the specific binding of [3H]InsP3 to isolated olfactory cilia, microsomes and brain membranes from the channel catfish (Ictalurus punctatus) and, by photoaffinity labelling with an InsP3 analogue (125I-labelled 1-[3-(4-azidosalicyloxy)-aminopropyl]inositol 1,4,5-trisphosphate (125I-ASA-InsP3)], we tentatively identify the major InsP3-binding protein in catfish olfactory cilia. InsP3 binding to ciliary membranes is specific and saturable, with a Kd of 1.10 +\/- 0.31 microM and a maximum number of binding sites (Bmax) of 17.6 +\/- 5.8 pmol\/mg. The rank order for potency of inhibition of [3H]InsP3 binding is Ins(1,4)P2 less than Ins(1,3,4)P3 less than Ins(1,3,4,5)P4 = Ins(1,4,5)P3 less than Ins(2,4,5)P3. Exposure of cilia membranes to u.v. light in the presence of 125I-ASA-InsP3 results in the labelling of a protein with apparent Mr 107,000. Labelling is specifically prevented by Ins(1,4,5)P3, Ins(2,4,5)P3 and Ins(1,3,4,5)P4, but not by Ins(1,4)P2 or Ins(1,3,4)P3. Both specific [3H]InsP3 binding and photoaffinity labelling of the Mr-107,000 protein were displaced by heparin. The Kd and the inhibition of [3H]InsP3 binding and of photoaffinity labelling by inositol phosphates and heparin are consistent with the ability of micromolar concentrations of Ins(1,4,5)P3 [but not Ins(1,3,4)P3] to activate the InsP3-gated currents in patch-clamp experiments with olfactory neurons. These results suggest that InsP3 binding to a Mr-107,000 cilia membrane protein may represent binding to the olfactory InsP3-gated cation channel.<\/jats:p>","DOI":"10.1042\/bj2810449","type":"journal-article","created":{"date-parts":[[2015,8,10]],"date-time":"2015-08-10T21:25:09Z","timestamp":1439241909000},"page":"449-456","update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":63,"title":["Characterization of a novel inositol 1,4,5-trisphosphate receptor in isolated olfactory cilia"],"prefix":"10.1042","volume":"281","author":[{"given":"D L","family":"Kalinoski","sequence":"first","affiliation":[{"name":"Monell Chemical Senses Center, 3500 Market Street, Philadelphia, PA 19104"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S B","family":"Aldinger","sequence":"additional","affiliation":[{"name":"Monell Chemical Senses Center, 3500 Market Street, Philadelphia, PA 19104"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A G","family":"Boyle","sequence":"additional","affiliation":[{"name":"Veterans Administration Medical Center, Philadelphia, PA 19104"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T","family":"Huque","sequence":"additional","affiliation":[{"name":"Monell Chemical Senses Center, 3500 Market Street, Philadelphia, PA 19104"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J F","family":"Marecek","sequence":"additional","affiliation":[{"name":"State University of New York, Department of Chemistry, Stony Brook, NY 11794, U.S.A."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G D","family":"Prestwich","sequence":"additional","affiliation":[{"name":"State University of New York, Department of Chemistry, Stony Brook, NY 11794, U.S.A."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D","family":"Restrepo","sequence":"additional","affiliation":[{"name":"Monell Chemical Senses Center, 3500 Market Street, Philadelphia, PA 19104"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","container-title":["Biochemical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/281\/2\/449\/605887\/bj2810449.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/281\/2\/449\/605887\/bj2810449.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,23]],"date-time":"2021-11-23T15:32:34Z","timestamp":1637681554000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/biochemj\/article\/281\/2\/449\/27331\/Characterization-of-a-novel-inositol-1-4-5"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1992,1,15]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1992,1,15]]}},"URL":"https:\/\/doi.org\/10.1042\/bj2810449","relation":{},"ISSN":["0264-6021","1470-8728"],"issn-type":[{"value":"0264-6021","type":"print"},{"value":"1470-8728","type":"electronic"}],"subject":[],"published":{"date-parts":[[1992,1,15]]}}}