{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T16:07:46Z","timestamp":1791130066838,"version":"4.1.0"},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"2","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1992,3,1]]},"abstract":"<jats:p>Hydroxyindole O-methyltransferase (EC 2.1.1.4) is the enzyme that catalyses the synthesis of melatonin in the pineal gland and in the retina. Polyadenylated RNA from chicken pineal glands was used to prepare a cDNA library in lambda gt11. The library was screened with an antiserum directed against chicken hydroxyindole O-methyltransferase, and one cDNA clone was isolated. The fusion protein expressed by phage lysogens was identified on Western blots as a 165 kDA immunoreactive protein (beta-galactosidase, 110 kDa; hydroxyindole O-methyltransferase, 38 kDa). The fusion protein exhibited hydroxyindole O-methyltransferase activity. Its Km values for N-acetyl-5-hydroxytryptamine and S-adenosylmethionine were 5 times those of the natural enzyme. The intrinsic activity of the fusion protein was approx. 0.25% that of the natural enzyme. The cDNA consisted of 1436 nucleotides, including a 1038-nucleotide sequence encoding a full-length 346-amino-acid hydroxyindole O-methyltransferase. Comparison with bovine hydroxyindole O-methyltransferase [Ishida, Obinata &amp; Deguchi (1987) J. Biol. Chem. 262, 2895-2899] revealed 52% identity in nucleotide sequences and 44% identity in peptide sequences. Northern-blot analysis revealed the presence of hydroxyindole O-methyltransferase mRNA transcripts in chicken pineal gland and retina, but not in the telencephalon.<\/jats:p>","DOI":"10.1042\/bj2820571","type":"journal-article","created":{"date-parts":[[2015,8,10]],"date-time":"2015-08-10T21:25:44Z","timestamp":1439241944000},"page":"571-576","update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":32,"title":["Molecular cloning and nucleotide sequence of a cDNA encoding hydroxyindole <i>O<\/i>-methyltransferase from chicken pineal gland"],"prefix":"10.1042","volume":"282","author":[{"given":"P","family":"Voisin","sequence":"first","affiliation":[{"name":"Laboratoire de Neurobiologie et Neuroendocrinologie Cellulaires, URA CNRS N\u00b0 290"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J","family":"Guerlott\u00e9","sequence":"additional","affiliation":[{"name":"Laboratoire de Neurobiologie et Neuroendocrinologie Cellulaires, URA CNRS N\u00b0 290"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M","family":"Bernard","sequence":"additional","affiliation":[{"name":"Laboratoire de Neurobiologie et Neuroendocrinologie Cellulaires, URA CNRS N\u00b0 290"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J P","family":"Collin","sequence":"additional","affiliation":[{"name":"Laboratoire de Neurobiologie et Neuroendocrinologie Cellulaires, URA CNRS N\u00b0 290"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M","family":"Cogn\u00e9","sequence":"additional","affiliation":[{"name":"Laboratoire d'Immunologie Mol\u00e9culaire, URA CNRS N\u00b0 1172, UFR Sciences, 40 Avenue du Recteur Pineau, 86022 Poitiers, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"288","container-title":["Biochemical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/282\/2\/571\/606671\/bj2820571.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/282\/2\/571\/606671\/bj2820571.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,23]],"date-time":"2021-11-23T21:50:53Z","timestamp":1637704253000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/biochemj\/article\/282\/2\/571\/27879\/Molecular-cloning-and-nucleotide-sequence-of-a"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1992,3,1]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1992,3,1]]}},"URL":"https:\/\/doi.org\/10.1042\/bj2820571","relation":{},"ISSN":["0264-6021","1470-8728"],"issn-type":[{"value":"0264-6021","type":"print"},{"value":"1470-8728","type":"electronic"}],"subject":[],"published":{"date-parts":[[1992,3,1]]}}}