{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,29]],"date-time":"2026-03-29T11:02:15Z","timestamp":1774782135920,"version":"3.50.1"},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"3","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1992,3,15]]},"abstract":"<jats:p>The metabolism of 5-aminosalicylate (5AS) by a bacterial strain, Pseudomonas sp. BN9, was studied. Intact cells of Pseudomonas sp. BN9 grown with 5AS oxidized 5AS and 2,5-dihydroxybenzoate (gentisate), whereas cells grown with gentisate oxidized only the growth substrate of all substituted salicylates tested. Cell extracts from Pseudomonas sp. BN9 catalysed the stoichiometric reaction of 1 mol of oxygen with 1 mol of 5AS to a metabolite with an intense u.v.-absorption maximum at 352 nm (pH 8.0). This metabolite was accumulated under neutral conditions, but was rapidly destroyed at acid pH. It was identified by m.s. and acid-catalysed deamination to fumarylpyruvate (trans-2,4-dioxohept-5-enedioic acid) as cis-4-amino-6-carboxy-2-oxohexa-3,5-dienoate, thus demonstrating direct cleavage of the monohydroxylated substrate 5AS to a non-aromatic ring-fission product. The enzyme responsible for conversion of 5AS was shown to be Fe(II)-dependent and to be distinct from gentisate 1,2-dioxygenase in strain BN9.<\/jats:p>","DOI":"10.1042\/bj2820675","type":"journal-article","created":{"date-parts":[[2015,8,10]],"date-time":"2015-08-10T21:25:44Z","timestamp":1439241944000},"page":"675-680","update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":27,"title":["Bacterial metabolism of 5-aminosalicylic acid. Initial ring cleavage"],"prefix":"10.1042","volume":"282","author":[{"given":"A","family":"Stolz","sequence":"first","affiliation":[{"name":"Institut f\u00fcr Mikrobiologie der Universit\u00e4t Stuttgart, Azenbergstrasse 18, 7 Stuttgart 1, Federal Republic of Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B","family":"N\u00f6rtemann","sequence":"additional","affiliation":[{"name":"Fachgebiet Chemie und Chemische Verfahrenstechnik, Universit\u00e4t Paderborn, Postfach 1621, 4790 Paderborn, Federal Republic of Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H J","family":"Knackmuss","sequence":"additional","affiliation":[{"name":"Institut f\u00fcr Mikrobiologie der Universit\u00e4t Stuttgart, Azenbergstrasse 18, 7 Stuttgart 1, Federal Republic of Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","container-title":["Biochemical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/282\/3\/675\/606210\/bj2820675.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/282\/3\/675\/606210\/bj2820675.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,23]],"date-time":"2021-11-23T21:48:19Z","timestamp":1637704099000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/biochemj\/article\/282\/3\/675\/27740\/Bacterial-metabolism-of-5-aminosalicylic-acid"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1992,3,15]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1992,3,15]]}},"URL":"https:\/\/doi.org\/10.1042\/bj2820675","relation":{},"ISSN":["0264-6021","1470-8728"],"issn-type":[{"value":"0264-6021","type":"print"},{"value":"1470-8728","type":"electronic"}],"subject":[],"published":{"date-parts":[[1992,3,15]]}}}