{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,3]],"date-time":"2026-03-03T05:08:35Z","timestamp":1772514515974,"version":"3.50.1"},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"2","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1992,6,1]]},"abstract":"<jats:p>Cross-linking of surface immunoglobulin (Ig) receptors on human B cells leads to the activation of a tyrosine kinase. The activated tyrosine kinase subsequently phosphorylates a number of substrates, including phospholipase C-gamma. This enzyme breaks down phosphoinositol bisphosphate to form two intracellular messengers, diacylglycerol and inositol 1,4,5-trisphosphate, leading to the activation of protein kinase C and the release of intracellular Ca2+ respectively. We have used h.p.l.c. and flow cytometry to measure accurately the inositol phosphate turnover and Ca2+ release in anti-Ig-stimulated human B cells. In particular, we have examined the effect of dose of the cross-linking antibody on the two responses. The identity of putative messenger inositol phosphates has been verified by structural analysis, and the amounts of both inositol phosphates and Ca2+ present have been quantified. In the Ramos Burkitt lymphoma, which is very sensitive to stimulus through its Ig receptors, both inositol phosphate production and Ca2+ release were found to be related to the dose of anti-Ig antibody applied. This suggests that phospholipase C-mediated signal transduction in human B cells converts the degree of cross-linking of the immunoglobulin receptor quantitatively into intracellular signals.<\/jats:p>","DOI":"10.1042\/bj2840447","type":"journal-article","created":{"date-parts":[[2015,8,13]],"date-time":"2015-08-13T21:20:57Z","timestamp":1439500857000},"page":"447-455","update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Relationships between the degree of cross-linking of surface immunoglobulin and the associated inositol 1,4,5-trisphosphate and Ca2+ signals in human B cells"],"prefix":"10.1042","volume":"284","author":[{"given":"F M","family":"McConnell","sequence":"first","affiliation":[{"name":"Regional Primate Research Center, University of Washington, Seattle, WA 98195, U.S.A."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S B","family":"Shears","sequence":"additional","affiliation":[{"name":"LCMP, NIEHS, P.O. Box 12233, Research Triangle Park, NC 27709, U.S.A."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P J L","family":"Lane","sequence":"additional","affiliation":[{"name":"Regional Primate Research Center, University of Washington, Seattle, WA 98195, U.S.A."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M S","family":"Scheibel","sequence":"additional","affiliation":[{"name":"Regional Primate Research Center, University of Washington, Seattle, WA 98195, U.S.A."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E A","family":"Clark","sequence":"additional","affiliation":[{"name":"Regional Primate Research Center, University of Washington, Seattle, WA 98195, U.S.A."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","container-title":["Biochemical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/284\/2\/447\/606976\/bj2840447.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/284\/2\/447\/606976\/bj2840447.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,23]],"date-time":"2021-11-23T21:55:57Z","timestamp":1637704557000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/biochemj\/article\/284\/2\/447\/28101\/Relationships-between-the-degree-of-cross-linking"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1992,6,1]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1992,6,1]]}},"URL":"https:\/\/doi.org\/10.1042\/bj2840447","relation":{},"ISSN":["0264-6021","1470-8728"],"issn-type":[{"value":"0264-6021","type":"print"},{"value":"1470-8728","type":"electronic"}],"subject":[],"published":{"date-parts":[[1992,6,1]]}}}