{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T07:49:39Z","timestamp":1782805779164,"version":"3.54.5"},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"1","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1992,7,1]]},"abstract":"<jats:p>beta-Oxidation of palmitate and tetradecanedioic acid was studied in cell-free extracts of the Gram-positive bacterium Corynebacterium sp. strain 7E1C, and the acyl-CoA ester intermediates formed were analysed by h.p.l.c. beta-Oxidation assays displayed a lag phase before a constant rate of NAD+ reduction was obtained. The length of the lag phase was inversely proportional to the number of units of activity added to assays. This is a characteristic feature of a system of consecutive reactions proceeding via free intermediates. During beta-oxidation of palmitate all the saturated acyl-CoAs from C16 to C8 were detected together with trace amounts of unsaturated and 3-hydroxy-intermediates. The time-course of intermediate formation again indicated a precursor-product relationship indicative of free intermediates being formed. When 3-hydroxyacyl-CoA dehydrogenase was inhibited by completely removing NAD+ from assays, the major acyl-CoAs, detected during palmitate beta-oxidation were palmitoyl-CoA, hexadeca-2-enoyl-CoA and 3-hydroxypalmitoyl-CoA. These compounds also displayed a precursor-product relationship. Under normal assay conditions the acyl-CoA dehydrogenase(s) are the probable rate-limiting enzyme(s) of the beta-oxidation spiral. These results indicate that in cell-free extracts of Corynebacterium sp. strain 7E1C, beta-oxidation proceeds via free acyl-CoA intermediates and is at variance with the concept of substrate channelling or of a \u2018leaky hose pipe\u2019 model as proposed for mitochondrial beta-oxidation in eukaryotic cells. The significant accumulation of chain-shortened acyl-CoA esters is similar to the situation observed for mammalian peroxisomal beta-oxidation.<\/jats:p>","DOI":"10.1042\/bj2850117","type":"journal-article","created":{"date-parts":[[2015,8,10]],"date-time":"2015-08-10T21:27:56Z","timestamp":1439242076000},"page":"117-122","update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Long-chain acyl-CoA ester intermediates of <i>\u03b2<\/i>-oxidation of mono- and di-carboxylic fatty acids by extracts of <i>Corynebacterium<\/i> sp. strain 7E1C"],"prefix":"10.1042","volume":"285","author":[{"given":"N M","family":"Broadway","sequence":"first","affiliation":[{"name":"Department of Applied Biology, University of Hull, Cottingham Road, Hull HU6 7RX, U.K."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F M","family":"Dickinson","sequence":"additional","affiliation":[{"name":"Department of Applied Biology, University of Hull, Cottingham Road, Hull HU6 7RX, U.K."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C","family":"Ratledge","sequence":"additional","affiliation":[{"name":"Department of Applied Biology, University of Hull, Cottingham Road, Hull HU6 7RX, U.K."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"288","container-title":["Biochemical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/285\/1\/117\/607645\/bj2850117.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/285\/1\/117\/607645\/bj2850117.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,23]],"date-time":"2021-11-23T22:17:55Z","timestamp":1637705875000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/biochemj\/article\/285\/1\/117\/29496\/Long-chain-acyl-CoA-ester-intermediates-of"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1992,7,1]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1992,7,1]]}},"URL":"https:\/\/doi.org\/10.1042\/bj2850117","relation":{},"ISSN":["0264-6021","1470-8728"],"issn-type":[{"value":"0264-6021","type":"print"},{"value":"1470-8728","type":"electronic"}],"subject":[],"published":{"date-parts":[[1992,7,1]]}}}