{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T18:36:13Z","timestamp":1760985373699},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"1","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1992,7,1]]},"abstract":"<jats:p>Methyl-lidocaine is an amphiphilic agent which has been used as an experimental anti-arrhythmic drug. When hamster hearts were perfused with labelled glycerol, the presence of methyl-lidocaine in the perfusate was found to enhance the labelling in phosphatidylserine, phosphatidylinositol, diacylglycerol and triacylglycerol. However, the labelling of phosphatidylcholine and phosphatidylethanolamine was not significantly changed by methyl-lidocaine treatment. Assays in vitro for the enzymes involved in the synthesis of neutral lipids and acidic phospholipids revealed that phosphatidate phosphatase and CTP: phosphatidate cytidylyltransferase activities were stimulated by methyl-lidocaine. The intracellular pool sizes of diacylglycerol and CDP-diacylglycerol were also elevated. We postulate that the enhanced syntheses of the neutral lipids and acidic phospholipids in the methyl-lidocaine-perfused heart were mediated via the direct activation of the key enzymes in the biosynthesis of these lipids de novo.<\/jats:p>","DOI":"10.1042\/bj2850161","type":"journal-article","created":{"date-parts":[[2015,8,10]],"date-time":"2015-08-10T21:27:56Z","timestamp":1439242076000},"page":"161-166","update-policy":"http:\/\/dx.doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["The effect of methyl-lidocaine on the biosynthesis of phospholipids <i>de novo<\/i> in the isolated hamster heart"],"prefix":"10.1042","volume":"285","author":[{"given":"P G","family":"Tardi","sequence":"first","affiliation":[{"name":"Department of Biochemistry & Molecular Biology and Pharmacology, Faculty of Medicine, University of Manitoba, Winnipeg, Manitoba, Canada R3E 0W3"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R Y K","family":"Man","sequence":"additional","affiliation":[{"name":"Department of Biochemistry & Molecular Biology and Pharmacology, Faculty of Medicine, University of Manitoba, Winnipeg, Manitoba, Canada R3E 0W3"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P C","family":"Choy","sequence":"additional","affiliation":[{"name":"Department of Biochemistry & Molecular Biology and Pharmacology, Faculty of Medicine, University of Manitoba, Winnipeg, Manitoba, Canada R3E 0W3"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","container-title":["Biochemical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/285\/1\/161\/607551\/bj2850161.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/285\/1\/161\/607551\/bj2850161.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,23]],"date-time":"2021-11-23T17:47:08Z","timestamp":1637689628000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/biochemj\/article\/285\/1\/161\/29361\/The-effect-of-methyl-lidocaine-on-the-biosynthesis"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1992,7,1]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1992,7,1]]}},"URL":"https:\/\/doi.org\/10.1042\/bj2850161","relation":{},"ISSN":["0264-6021","1470-8728"],"issn-type":[{"value":"0264-6021","type":"print"},{"value":"1470-8728","type":"electronic"}],"subject":[],"published":{"date-parts":[[1992,7,1]]}}}