{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T23:16:04Z","timestamp":1789514164535,"version":"build-2803163510"},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"3","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1992,11,1]]},"abstract":"<jats:p>A tachykinin peptide was isolated from an extract of the intestine of the European green frog, Rana ridibunda, and its primary structure was established as: His-Lys-Leu-Asp-Ser-Phe-Ile-Gly-Leu-Met.CONH2. This sequence was confirmed by chemical synthesis and shows two amino acid substitutions (leucine for threonine at position 3 and isoleucine for valine at position 7) compared with neurokinin A. Binding parameters for synthetic [Leu3, Ile7]neurokinin A and mammalian tachykinins were compared using receptor-selective radioligands and crude membranes from tissues enriched in the NK1, NK2 and NK3 receptors. [Leu3, Ile7]Neurokinin A was approx. 3-fold less potent than substance P in inhibiting the binding of 125I-labelled [Sar9, Met(O2)11]substance P (labelled with Bolton-Hunter reagent) to rat submandibular gland (NK1 receptor), 8-fold less potent than neurokinin A in inhibiting the binding of [2-[125I]iodohistidine1]neurokinin A to rat stomach fundus (NK2 receptor) and 6-fold less potent than neurokinin B in inhibiting the binding of 125I-Bolton-Hunter-labelled scyliorhinin II to rat brain (NK3 receptor). Thus the frog neurokinin A-related peptide shows moderate affinity but lack of selectivity for all three tachykinin-binding sites in rat tissues. This non-selectivity is similar to that displayed by the molluscan tachykinin, eledoisin, which also contains an isoleucine residue in the corresponding position in the molecule.<\/jats:p>","DOI":"10.1042\/bj2870827","type":"journal-article","created":{"date-parts":[[2015,8,10]],"date-time":"2015-08-10T21:28:56Z","timestamp":1439242136000},"page":"827-832","update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["Primary structure and receptor-binding properties of a neurokinin A-related peptide from frog gut"],"prefix":"10.1042","volume":"287","author":[{"given":"Y","family":"Wang","sequence":"first","affiliation":[{"name":"Regulatory Peptide Center, Department of Biomedical Sciences, Creighton University Medical School, Omaha, NE 68178, U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T","family":"Badgery-Parker","sequence":"additional","affiliation":[{"name":"School of Physiology and Pharmacology, University of New South Wales, Sydney, 2033 Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S","family":"Lovas","sequence":"additional","affiliation":[{"name":"Regulatory Peptide Center, Department of Biomedical Sciences, Creighton University Medical School, Omaha, NE 68178, U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N","family":"Chartrel","sequence":"additional","affiliation":[{"name":"European Institute for Peptide Research, Laboratory for Molecular Endocrinology, C.N.R.S. URA 650, INSERM, University of Rouen, 76134 Mont-Saint-Aignan, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H","family":"Vaudry","sequence":"additional","affiliation":[{"name":"European Institute for Peptide Research, Laboratory for Molecular Endocrinology, C.N.R.S. URA 650, INSERM, University of Rouen, 76134 Mont-Saint-Aignan, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E","family":"Burcher","sequence":"additional","affiliation":[{"name":"School of Physiology and Pharmacology, University of New South Wales, Sydney, 2033 Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J M","family":"Conlon","sequence":"additional","affiliation":[{"name":"Regulatory Peptide Center, Department of Biomedical Sciences, Creighton University Medical School, Omaha, NE 68178, U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"288","container-title":["Biochemical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/287\/3\/827\/628919\/bj2870827.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/287\/3\/827\/628919\/bj2870827.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,23]],"date-time":"2021-11-23T22:10:24Z","timestamp":1637705424000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/biochemj\/article\/287\/3\/827\/28750\/Primary-structure-and-receptor-binding-properties"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1992,11,1]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1992,11,1]]}},"URL":"https:\/\/doi.org\/10.1042\/bj2870827","relation":{},"ISSN":["0264-6021","1470-8728"],"issn-type":[{"value":"0264-6021","type":"print"},{"value":"1470-8728","type":"electronic"}],"subject":[],"published":{"date-parts":[[1992,11,1]]}}}