{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T22:28:04Z","timestamp":1762295284946},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"2","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1992,12,1]]},"abstract":"<jats:p>Cytochrome P-450 was purified to apparent homogeneity from the brain microsomes of phenobarbital-treated rats. The specific content of the purified P-450 was 12.7 nmol\/mg of protein. NADPH-cytochrome P-450 reductase (reductase) was also purified to apparent homogeneity from brain microsomes. The specific content was 34.7 mumol of cytochrome c reduced\/min per mg of protein. The reduced carbon monoxide spectrum of purified P-450 exhibited a peak at 450 nm. Both the P-450 and the reductase moved as single bands on SDS\/PAGE. The molecular masses of the purified P-450 and the reductase were determined to be 53.3 and 72.0 kDa respectively. The purified brain P-450 cross-reacted with antibodies to rat liver P-450IIB1\/IIB2 when examined by Western immunoblotting, but no immunological similarity was observed with rat liver P-450IA1\/IA2 or P-450IIE1. Purified rat brain reductase cross-reacted with antibodies to rat liver reductase. Further, immunoblot experiments with untreated rat and human brain microsomes using antisera to the purified rat brain P-450 and reductase indicated that these forms of P-450 and NADPH-cytochrome P-450 reductase exist constitutively in rat and human brain. Purified rat brain P-450 was reconstituted with purified NADPH-cytochrome P-450 reductase, deoxycholate and dilauroyl glyceryl 3-phosphocholine. NADPH-dependent N-demethylation of aminopyrine and morphine was observed in the reconstituted system. The catalytic-centre activities were 80.25 and 38.2 nmol of formaldehyde formed\/min per nmol of P-450 respectively. The reconstituted system had a comparatively lower catalytic-centre activity for 7-ethoxycoumarin O-de-ethylase (10.5 nmol of product formed\/min per nmol of P-450).<\/jats:p>","DOI":"10.1042\/bj2880483","type":"journal-article","created":{"date-parts":[[2015,8,10]],"date-time":"2015-08-10T21:29:52Z","timestamp":1439242192000},"page":"483-488","update-policy":"http:\/\/dx.doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Characterization of a phenobarbital-inducible cytochrome <i>P<\/i>-450, NADPH-cytochrome <i>P<\/i>-450 reductase and reconstituted cytochrome <i>P<\/i>-450 mono-oxygenase system from rat brain. Evidence for constitutive presence in rat and human brain"],"prefix":"10.1042","volume":"288","author":[{"given":"H K","family":"Anandatheerthavarada","sequence":"first","affiliation":[{"name":"Department of Neurochemistry, National Institute of Mental Health and Neuro Sciences, Hosur Road, Bangalore-560029, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M R","family":"Boyd","sequence":"additional","affiliation":[{"name":"Laboratory of Drug Discovery Reseach and Development, National Cancer Institute, National Institutes of Health, Frederick, MD 21702-1201, U.S.A."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"V","family":"Ravindranath","sequence":"additional","affiliation":[{"name":"Department of Neurochemistry, National Institute of Mental Health and Neuro Sciences, Hosur Road, Bangalore-560029, India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","container-title":["Biochemical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/288\/2\/483\/608583\/bj2880483.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/288\/2\/483\/608583\/bj2880483.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,23]],"date-time":"2021-11-23T22:13:23Z","timestamp":1637705603000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/biochemj\/article\/288\/2\/483\/28882\/Characterization-of-a-phenobarbital-inducible"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1992,12,1]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1992,12,1]]}},"URL":"https:\/\/doi.org\/10.1042\/bj2880483","relation":{},"ISSN":["0264-6021","1470-8728"],"issn-type":[{"value":"0264-6021","type":"print"},{"value":"1470-8728","type":"electronic"}],"subject":[],"published":{"date-parts":[[1992,12,1]]}}}