{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T22:07:41Z","timestamp":1787004461456,"version":"build-2736575974"},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"3","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1993,12,15]]},"abstract":"<jats:p>The metabolic fate of the anticoagulant protein, hirudin, and its complex with thrombin are presently unknown. Therefore we have labelled hirudin and human thrombin-hirudin complex with the residualizing label dilactitol-125I-tyramine (*I-DLT) in order to identify their tissue sites of catabolism in the rat. The rapid plasma clearance of hirudin after intravenous injection was unaffected by *I-DLT labelling, and by 2 h 6% or less of the injected dose remained in the blood. The majority (80.3 +\/- 4.0%, n = 2) of *I-DLT-hirudin radioactivity recovered in tissues was found in kidney, and kidney was also at least 150 times more active in taking up hirudin, on a weight basis, than any other tissue examined (liver, spleen, skin, muscle, intestine, fat, lung). *I-DLT-hirudin which bound to thrombin was isolated by chromatography on concanavalin A-Sepharose; hirudin itself does not bind to concanavalin A. Radioactivity from thrombin-*I-DLT-hirudin was precipitable by anti-thrombin antibody and *I-DLT-thrombin-hirudin was precipitable by anti-hirudin antibody. By 1 h after injection of labelled thrombin-hirudin complexes, the recoveries of radioactivity from hirudin and thrombin in liver were comparable (38.6 +\/- 3.0 and 36.4 +\/- 4.1%, n = 3), whereas more radioactivity was recovered in kidney from hirudin than from thrombin (27.6 +\/- 8.7 compared with 13.6 +\/- 4.5%) and less was recovered in lung (0.4 +\/- 0.2 compared with 17.7 +\/- 2.9%). We conclude that hirudin is catabolized predominantly in kidney, whereas the thrombin-hirudin complex is catabolized by both liver and kidney.<\/jats:p>","DOI":"10.1042\/bj2960771","type":"journal-article","created":{"date-parts":[[2015,8,10]],"date-time":"2015-08-10T17:35:38Z","timestamp":1439228138000},"page":"771-776","update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Catabolism of hirudin and thrombin-hirudin complexes in the rat"],"prefix":"10.1042","volume":"296","author":[{"given":"J","family":"Bichler","sequence":"first","affiliation":[{"name":"Department of Chemistry and Biochemistry, University of South Carolina, Columbia, SC 29208, U.S.A."},{"name":"Department of Clinical Chemistry and Clinical Biochemistry in the Surgical Clinic, University of Munich, Munich, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J W","family":"Baynes","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biochemistry, University of South Carolina, Columbia, SC 29208, U.S.A."},{"name":"School of Medicine, University of South Carolina, Columbia, SC 29208, U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S R","family":"Thorpe","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biochemistry, University of South Carolina, Columbia, SC 29208, U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"288","container-title":["Biochemical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/296\/3\/771\/612161\/bj2960771.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/296\/3\/771\/612161\/bj2960771.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,23]],"date-time":"2021-11-23T18:16:07Z","timestamp":1637691367000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/biochemj\/article\/296\/3\/771\/31375\/Catabolism-of-hirudin-and-thrombin-hirudin"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1993,12,15]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1993,12,15]]}},"URL":"https:\/\/doi.org\/10.1042\/bj2960771","relation":{},"ISSN":["0264-6021","1470-8728"],"issn-type":[{"value":"0264-6021","type":"print"},{"value":"1470-8728","type":"electronic"}],"subject":[],"published":{"date-parts":[[1993,12,15]]}}}