{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,9,17]],"date-time":"2023-09-17T04:54:03Z","timestamp":1694926443101},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"1","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1994,1,1]]},"abstract":"<jats:p>Transducin alpha (T alpha) activates retinal rod cyclic GMP phosphodiesterase (PDE) by interacting with and removing the inhibitory PDE gamma subunit. A T alpha-PDE gamma complex can be isolated in vitro, and our previous work [Morrison, Rider and Takemoto (1987) FEBS Lett. 222, 266-270; Morrison, Cunnick, Oppert and Takemoto (1989) J. Biol. Chem. 264, 11671-11681] has identified a region of PDE gamma, residues 24-45, that binds to T alpha. The C-terminal region of PDE gamma is the site that interacts with PDE alpha\/beta and inhibits catalytic function. The site on T alpha that binds to the PDE gamma 24-45 region has not been identified. Synthetic peptides (15-mers) which span the bovine T alpha sequence were tested for binding to purified recombinant PDE gamma using a solid-phase assay. The peptides were also tested for ability to activate a PDE complex. We have identified a region, residues 250-275 of T alpha, which shows a high affinity of PDE gamma and for the PDE gamma (24-45) binding peptide. The peptide did not bind to the C-terminal residues 50-87 of PDE gamma. Likewise, a region of T alpha, 1-25 did not exhibit high-affinity binding to PDE gamma or to the 24-45 PDE gamma peptide. Specific binding of the 250-275 peptide to PDE gamma was confirmed by its ability to compete with T alpha binding to PDE gamma, although a higher concentration was required (10x). The T alpha-(250-275) peptide activated a fully inhibited PDE alpha beta gamma 2 complex in a dose-dependent manner. These results suggest that a region on T alpha that recognizes the PDE gamma-binding site is found within residues 250-275 of T alpha.<\/jats:p>","DOI":"10.1042\/bj2970087","type":"journal-article","created":{"date-parts":[[2015,8,10]],"date-time":"2015-08-10T21:36:00Z","timestamp":1439242560000},"page":"87-91","update-policy":"http:\/\/dx.doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Identification of a binding site on retinal transducin <i>\u03b1<\/i> for the phosphodiesterase inhibitory <i>\u03b3<\/i> subunit"],"prefix":"10.1042","volume":"297","author":[{"given":"J","family":"Cunnick","sequence":"first","affiliation":[{"name":"Department of Biochemistry, Kansas State University, Manhattan, KS 66506, U.S.A."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C","family":"Twamley","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Kansas State University, Manhattan, KS 66506, U.S.A."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"I","family":"Udovichenko","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Kansas State University, Manhattan, KS 66506, U.S.A."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K","family":"Gonzalez","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Kansas State University, Manhattan, KS 66506, U.S.A."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D J","family":"Takemoto","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Kansas State University, Manhattan, KS 66506, U.S.A."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","container-title":["Biochemical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/297\/1\/87\/612524\/bj2970087.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/297\/1\/87\/612524\/bj2970087.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,23]],"date-time":"2021-11-23T20:12:10Z","timestamp":1637698330000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/biochemj\/article\/297\/1\/87\/30067\/Identification-of-a-binding-site-on-retinal"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1994,1,1]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1994,1,1]]}},"URL":"https:\/\/doi.org\/10.1042\/bj2970087","relation":{},"ISSN":["0264-6021","1470-8728"],"issn-type":[{"value":"0264-6021","type":"print"},{"value":"1470-8728","type":"electronic"}],"subject":[],"published":{"date-parts":[[1994,1,1]]}}}