{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T19:59:49Z","timestamp":1760299189762},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"2","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1994,1,15]]},"abstract":"<jats:p>FeMoco, a low-M(r) metal cluster of probable composition Fe7MoS9 complexed with homocitrate, has been extracted with N-methylformamide from the MoFe protein of the nitrogenase enzyme from Klebsiella pneumoniae. The binding of cyanide and thiols to the FeMoco cluster in its paramagnetic S = 3\/2 oxidation level has been studied by low-temperature e.p.r. and magnetic-circular-dichroism (m.c.d.) spectroscopies. Cyanide binds to isolated FeMoco at more than one site, and causes changes in the g values form g = 4.6, 3.2, 2.0 to g = 4.29, 3.82, 2.02 E.p.r. competition studies indicate that one cyanide can be displaced by thiolate from one type of site. The form of the low-temperature m.c.d. spectrum is little changed by ligand binding, thus the basic cluster structure remains intact. However, when benzenethiol is bound, a new intense band (lambda 387 nm) is observed, indicating the generation of an increased ligand-to-cluster charge-transfer interaction.<\/jats:p>","DOI":"10.1042\/bj2970373","type":"journal-article","created":{"date-parts":[[2015,8,10]],"date-time":"2015-08-10T21:36:00Z","timestamp":1439242560000},"page":"373-378","update-policy":"http:\/\/dx.doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Electron-paramagnetic-resonance and magnetic-circular-dichroism studies of the binding of cyanide and thiols to the iron-molybdenum cofactor from <i>Klebsiella pneumoniae<\/i> nitrogenase"],"prefix":"10.1042","volume":"297","author":[{"given":"A J M","family":"Richards","sequence":"first","affiliation":[{"name":"School of Chemical Sciences, University of East Anglia, Norwich NR4 7TJ, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D J","family":"Lowe","sequence":"additional","affiliation":[{"name":"AFRC Institute of Plant Science Research, Nitrogen Fixation Laboratory, University of Sussex, Brighton BN1 9RQ, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R L","family":"Richards","sequence":"additional","affiliation":[{"name":"AFRC Institute of Plant Science Research, Nitrogen Fixation Laboratory, University of Sussex, Brighton BN1 9RQ, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A J","family":"Thomson","sequence":"additional","affiliation":[{"name":"School of Chemical Sciences, University of East Anglia, Norwich NR4 7TJ, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B E","family":"Smith","sequence":"additional","affiliation":[{"name":"AFRC Institute of Plant Science Research, Nitrogen Fixation Laboratory, University of Sussex, Brighton BN1 9RQ, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","container-title":["Biochemical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/297\/2\/373\/724030\/bj2970373.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/297\/2\/373\/724030\/bj2970373.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,23]],"date-time":"2021-11-23T22:21:44Z","timestamp":1637706104000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/biochemj\/article\/297\/2\/373\/29995\/Electron-paramagnetic-resonance-and-magnetic"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1994,1,15]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1994,1,15]]}},"URL":"https:\/\/doi.org\/10.1042\/bj2970373","relation":{},"ISSN":["0264-6021","1470-8728"],"issn-type":[{"value":"0264-6021","type":"print"},{"value":"1470-8728","type":"electronic"}],"subject":[],"published":{"date-parts":[[1994,1,15]]}}}