{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,27]],"date-time":"2025-10-27T05:01:20Z","timestamp":1761541280473},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"1","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1994,2,15]]},"abstract":"<jats:p>Platelet aggregation and stimulation of phosphoinositide-specific phospholipase C (PLC) by thrombin and by convulxin (Cvx), a non-enzymic snake venom glycoprotein, were compared. Cvx-stimulated production of inositol phosphates by washed platelets was independent of the cyclo-oxygenase pathway, formation of platelet-activating factor and ADP release, but prostacyclin (prostaglandin I2), a stimulator of cyclic AMP formation, suppressed its effects on platelet and PLC activation. Kinetic analysis showed that inositol 1,4,5-trisphosphate formation reached its maximal value 15 s after platelet stimulation with Cvx and persisted for at least 5 min. Neomycin sulphate (10 mM), which complexes phosphatidylinositol 4-phosphate and phosphatidyl-inositol 4,5-bisphosphate, decreased the production of inositol phosphates, partially prevented platelet aggregation induced by a high concentration of Cvx (10 nM) and abolished both platelet aggregation and inositol phosphate formation induced by thrombin (2 units\/ml) and by a stable prostaglandin H2 analogue, U46619 (1 microM). In contrast with neomycin sulphate, Na2SO4 had no significant effect against all agonists tested. It is concluded that platelet activation by Cvx is partially mediated by PLC and involves other mechanisms as well.<\/jats:p>","DOI":"10.1042\/bj2980087","type":"journal-article","created":{"date-parts":[[2015,8,10]],"date-time":"2015-08-10T21:36:28Z","timestamp":1439242588000},"page":"87-91","update-policy":"http:\/\/dx.doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":25,"title":["Convulxin-induced platelet aggregation is accompanied by a powerful activation of the phospholipase C pathway"],"prefix":"10.1042","volume":"298","author":[{"given":"A","family":"Faili","sequence":"first","affiliation":[{"name":"Unit\u00e9 de Pharmacologie Cellulaire, Unit\u00e9 Associ\u00e9e Institut Pasteur-INSERM U285, Paris, France."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J","family":"Randon","sequence":"additional","affiliation":[{"name":"Unit\u00e9 de Pharmacologie Cellulaire, Unit\u00e9 Associ\u00e9e Institut Pasteur-INSERM U285, Paris, France."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"I M","family":"Francischetti","sequence":"additional","affiliation":[{"name":"Unit\u00e9 des Venins, Institut Pasteur, 25 rue du Dr. Roux, 75015 Paris, France."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B B","family":"Vargaftig","sequence":"additional","affiliation":[{"name":"Unit\u00e9 de Pharmacologie Cellulaire, Unit\u00e9 Associ\u00e9e Institut Pasteur-INSERM U285, Paris, France."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M","family":"Hatmi","sequence":"additional","affiliation":[{"name":"Unit\u00e9 de Pharmacologie Cellulaire, Unit\u00e9 Associ\u00e9e Institut Pasteur-INSERM U285, Paris, France."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","container-title":["Biochemical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/298\/1\/87\/612744\/bj2980087.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/298\/1\/87\/612744\/bj2980087.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,23]],"date-time":"2021-11-23T22:00:01Z","timestamp":1637704801000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/biochemj\/article\/298\/1\/87\/30450\/Convulxin-induced-platelet-aggregation-is"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1994,2,15]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1994,2,15]]}},"URL":"https:\/\/doi.org\/10.1042\/bj2980087","relation":{},"ISSN":["0264-6021","1470-8728"],"issn-type":[{"value":"0264-6021","type":"print"},{"value":"1470-8728","type":"electronic"}],"subject":[],"published":{"date-parts":[[1994,2,15]]}}}