{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,20]],"date-time":"2025-09-20T21:15:46Z","timestamp":1758402946967},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"1","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1994,2,15]]},"abstract":"<jats:p>The effect of alpha-actinin from chicken gizzard on the properties of F-actin solutions at 37 degrees C and at 4 degrees C was investigated. Beside the well-known increase of the gelling activity of alpha-actinin, it was found that lowering temperature to 4 degrees C: (a) modifies the shape of the alpha-actinin-F-actin binding isotherm; (b) increases the light scattering of the alpha-actinin-F-actin mixtures; (c) induces the formation of ribbons and bundles of F-actin. It was also observed that, by warming to 37 degrees C, the bundles of F-actin formed at 4 degrees C were dissociated into quasi-parallel actin filaments running at a distance of 25-42 nm from each other. On subsequent cooling to 4 degrees C, these parallel filaments were rapidly assembled into bundles. As at 37 degrees C, alpha-actinin displays a potent gelling activity on bundles but not on filaments of actin, and as the gelling activity at 4 degrees C is accompanied by the formation of actin bundles, it is concluded that actin bundling is a necessary condition to promote the actin-gelling activity.<\/jats:p>","DOI":"10.1042\/bj2980129","type":"journal-article","created":{"date-parts":[[2015,8,10]],"date-time":"2015-08-10T21:36:28Z","timestamp":1439242588000},"page":"129-133","update-policy":"http:\/\/dx.doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["<i>\u03b1<\/i>-Actinin from chicken gizzard: at low temperature, the onset of actin-gelling activity correlates with actin bundling"],"prefix":"10.1042","volume":"298","author":[{"given":"E","family":"Grazi","sequence":"first","affiliation":[{"name":"Istituto di Chimica Biologica, Universit\u00e0 di Ferrara, Via Borsari 46, 44100 Ferrara, Italy."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G","family":"Trombetta","sequence":"additional","affiliation":[{"name":"Istituto di Chimica Biologica, Universit\u00e0 di Ferrara, Via Borsari 46, 44100 Ferrara, Italy."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E","family":"Magri","sequence":"additional","affiliation":[{"name":"Istituto di Chimica Biologica, Universit\u00e0 di Ferrara, Via Borsari 46, 44100 Ferrara, Italy."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P","family":"Cuneo","sequence":"additional","affiliation":[{"name":"Istituto di Chimica Biologica, Universit\u00e0 di Ferrara, Via Borsari 46, 44100 Ferrara, Italy."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C","family":"Schwienbacher","sequence":"additional","affiliation":[{"name":"Istituto di Chimica Biologica, Universit\u00e0 di Ferrara, Via Borsari 46, 44100 Ferrara, Italy."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","container-title":["Biochemical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/298\/1\/129\/612716\/bj2980129.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/298\/1\/129\/612716\/bj2980129.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,23]],"date-time":"2021-11-23T21:59:27Z","timestamp":1637704767000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/biochemj\/article\/298\/1\/129\/30447\/Actinin-from-chicken-gizzard-at-low-temperature"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1994,2,15]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1994,2,15]]}},"URL":"https:\/\/doi.org\/10.1042\/bj2980129","relation":{},"ISSN":["0264-6021","1470-8728"],"issn-type":[{"value":"0264-6021","type":"print"},{"value":"1470-8728","type":"electronic"}],"subject":[],"published":{"date-parts":[[1994,2,15]]}}}