{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T15:01:42Z","timestamp":1791126102944,"version":"4.1.0"},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"1","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1994,5,15]]},"abstract":"<jats:p>Asp-130 of the recombinant skeletal-muscle 6-phosphofructo-2-kinase (PFK-2)\/fructose-2,6-bisphosphatase was mutated into Ala in order to study its role in catalysis and\/or substrate binding. The D130A mutant displayed a 30- to 140-fold decreased 2-kinase Vmax, depending on the pH, and a 30- and 60-fold increase in Km for MgATP and Fru-6-P respectively at pH 8.5 compared with the wild-type. Mutagenesis of Asp-130 to Ala had no effect on the 2-phosphatase activity, and fluorescence measurements indicated that the changes in kinetic properties of PFK-2 in the D130A mutant were not due to instability. The role of Asp-130 in the 2-kinase reaction is discussed and compared with that of Asp-103 of 6-phosphofructo-1-kinase from Escherichia coli, which binds Mg2+.<\/jats:p>","DOI":"10.1042\/bj3000111","type":"journal-article","created":{"date-parts":[[2015,8,10]],"date-time":"2015-08-10T21:37:53Z","timestamp":1439242673000},"page":"111-115","update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Site-directed mutagenesis of rat muscle 6-phosphofructo-2-kinase\/fructose-2,6-bisphosphatase: role of Asp-130 in the 2-kinase domain"],"prefix":"10.1042","volume":"300","author":[{"given":"M H","family":"Rider","sequence":"first","affiliation":[{"name":"Hormone and Metabolic Research Unit, International Institute of Cellular and Molecular Pathology and University of Louvain Medical School, Avenue Hippocrate, 75, B-1200 Brussels, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K M","family":"Crepin","sequence":"additional","affiliation":[{"name":"Hormone and Metabolic Research Unit, International Institute of Cellular and Molecular Pathology and University of Louvain Medical School, Avenue Hippocrate, 75, B-1200 Brussels, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M","family":"De Cloedt","sequence":"additional","affiliation":[{"name":"Hormone and Metabolic Research Unit, International Institute of Cellular and Molecular Pathology and University of Louvain Medical School, Avenue Hippocrate, 75, B-1200 Brussels, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L","family":"Bertrand","sequence":"additional","affiliation":[{"name":"Hormone and Metabolic Research Unit, International Institute of Cellular and Molecular Pathology and University of Louvain Medical School, Avenue Hippocrate, 75, B-1200 Brussels, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L","family":"Hue","sequence":"additional","affiliation":[{"name":"Hormone and Metabolic Research Unit, International Institute of Cellular and Molecular Pathology and University of Louvain Medical School, Avenue Hippocrate, 75, B-1200 Brussels, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"288","container-title":["Biochemical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/300\/1\/111\/613485\/bj3000111.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/300\/1\/111\/613485\/bj3000111.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,23]],"date-time":"2021-11-23T22:49:12Z","timestamp":1637707752000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/biochemj\/article\/300\/1\/111\/30769\/Site-directed-mutagenesis-of-rat-muscle-6"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1994,5,15]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1994,5,15]]}},"URL":"https:\/\/doi.org\/10.1042\/bj3000111","relation":{},"ISSN":["0264-6021","1470-8728"],"issn-type":[{"value":"0264-6021","type":"print"},{"value":"1470-8728","type":"electronic"}],"subject":[],"published":{"date-parts":[[1994,5,15]]}}}