{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T11:35:39Z","timestamp":1789990539483,"version":"4.0.1"},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"3","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1994,6,15]]},"abstract":"<jats:p>7-Hydroxymethotrexate (7-OH-MTX) is the major and, frequently the only, pteridine metabolite found in bone-marrow aspirates of patients chronically treated with low-dose oral methotrexate (MTX) [Sonneveld, Schultz, Nooter and Hahlen (1986) Cancer Chemother. Pharmacol. 18, 111-116]. The Ki values for MTX and 7-OH-MTX for avian liver 5-amino-imidazole-4-carboxamide ribonucleotide transformylase differ by 4.5-fold in favour of 7-OH-MTX as the better inhibitor, while Ki values for avian liver glycinamide ribonucleotide transformylase differ by 1.9-fold favouring MTX as the better inhibitor. Thus 7-OH-MTX possesses a different enzyme-inhibiting repertoire from its parent drug and this information may be useful in explaining the mechanism of action of low-dose MTX therapies used to treat autoimmune disease.<\/jats:p>","DOI":"10.1042\/bj3000627","type":"journal-article","created":{"date-parts":[[2015,8,10]],"date-time":"2015-08-10T21:37:53Z","timestamp":1439242673000},"page":"627-629","update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Differences in methotrexate and 7-hydroxymethotrexate inhibition of folate-dependent enzymes of purine nucleotide biosynthesis"],"prefix":"10.1042","volume":"300","author":[{"given":"J E","family":"Baggott","sequence":"first","affiliation":[{"name":"Department of Nutrition Sciences, University of Alabama at Birmingham, UAB Station, Birmingham, AL 35294, U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S L","family":"Morgan","sequence":"additional","affiliation":[{"name":"Department of Nutrition Sciences, University of Alabama at Birmingham, UAB Station, Birmingham, AL 35294, U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"W H","family":"Vaughn","sequence":"additional","affiliation":[{"name":"Department of Nutrition Sciences, University of Alabama at Birmingham, UAB Station, Birmingham, AL 35294, U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"288","container-title":["Biochemical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/300\/3\/627\/613977\/bj3000627.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/300\/3\/627\/613977\/bj3000627.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,23]],"date-time":"2021-11-23T23:05:01Z","timestamp":1637708701000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/biochemj\/article\/300\/3\/627\/30961\/Differences-in-methotrexate-and-7"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1994,6,15]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1994,6,15]]}},"URL":"https:\/\/doi.org\/10.1042\/bj3000627","relation":{},"ISSN":["0264-6021","1470-8728"],"issn-type":[{"value":"0264-6021","type":"print"},{"value":"1470-8728","type":"electronic"}],"subject":[],"published":{"date-parts":[[1994,6,15]]}}}