{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,5]],"date-time":"2025-10-05T16:59:26Z","timestamp":1759683566342},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"3","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1994,8,1]]},"abstract":"<jats:p>The effects of caffeine on agonist-induced changes in intracellular Ca2+ concentration ([Ca2+]i) were studied in single fura 2-loaded cells and suspensions of rat hepatocytes. In single cells, caffeine (5-10 mM) inhibited [Ca2+]i oscillations induced both by noradrenaline (0.1 microM) and by vasopressin (0.1 nM). Caffeine shifted the dose-response curves of the [Ca2+]i rise induced by vasopressin (0.5 to 2 nM) and noradrenaline (from 80 to 580 nM) in suspensions of liver cells loaded with quin2. This inhibitory effect of caffeine was not due to inhibition of phosphodiesterase enzymes and elevation of cyclic AMP levels, because application of 3-isobutyl-1-methylxanthine, forskolin or 8-bromo cyclic AMP had no inhibitory effect on the intracellular Ca2+ rise induced by inositol 1,4,5-trisphosphate (InsP3)-dependent agonists. We demonstrate that the inhibitory effect of caffeine may result from at least three actions of caffeine: (1) inhibition of receptor-stimulated InsP3 formation; (2) inhibition of agonist-stimulated Ca2+ influx; and (3) direct inhibition of the InsP3-sensitive Ca(2+)-release channel.<\/jats:p>","DOI":"10.1042\/bj3010737","type":"journal-article","created":{"date-parts":[[2015,8,10]],"date-time":"2015-08-10T21:38:33Z","timestamp":1439242713000},"page":"737-744","update-policy":"http:\/\/dx.doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Caffeine inhibits cytosolic calcium oscillations induced by noradrenaline and vasopressin in rat hepatocytes"],"prefix":"10.1042","volume":"301","author":[{"given":"L","family":"Combettes","sequence":"first","affiliation":[{"name":"Unit\u00e9 de Recherche U274, Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale, Universit\u00e9 Paris Sud, bat. 443, 91405 Orsay, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B","family":"Berthon","sequence":"additional","affiliation":[{"name":"Unit\u00e9 de Recherche U274, Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale, Universit\u00e9 Paris Sud, bat. 443, 91405 Orsay, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M","family":"Claret","sequence":"additional","affiliation":[{"name":"Unit\u00e9 de Recherche U274, Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale, Universit\u00e9 Paris Sud, bat. 443, 91405 Orsay, France"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","container-title":["Biochemical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/301\/3\/737\/613776\/bj3010737.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/301\/3\/737\/613776\/bj3010737.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,23]],"date-time":"2021-11-23T23:15:36Z","timestamp":1637709336000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/biochemj\/article\/301\/3\/737\/31334\/Caffeine-inhibits-cytosolic-calcium-oscillations"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1994,8,1]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1994,8,1]]}},"URL":"https:\/\/doi.org\/10.1042\/bj3010737","relation":{},"ISSN":["0264-6021","1470-8728"],"issn-type":[{"value":"0264-6021","type":"print"},{"value":"1470-8728","type":"electronic"}],"subject":[],"published":{"date-parts":[[1994,8,1]]}}}