{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,24]],"date-time":"2025-10-24T13:04:20Z","timestamp":1761311060264},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"2","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1994,9,1]]},"abstract":"<jats:p>The thermodynamics of binding of fluoride and cyanide to cytochrome c peroxidase (CCP) and its Asn-82--&amp;gt;Asp mutant (D82CCP) in phosphate and acetate buffer at an ionic strength of 0.15 mol.kg-1 from pH 5.0 to 7.1 were investigated by titration calorimetry at 289 and 297 K. The binding reactions are enthalpically driven. The fluoride-binding constants determined from the titration calorimetry results were in agreement with those determined from difference-spectroscopy measurements. For cyanide binding to CCP at 297.9 K, the binding constant decreased from 8.95 (+\/- 0.83) x 10(5) M-1 at pH 7.0 to 4.04(+\/- 0.23) x 10(5) M-1 at pH 5.0, and the binding enthalpy increased from -57.2 +\/- 1.4 kJ.mol-1 at pH 7.0 to -48.6 +\/- 1.8 kJ.mol-1 at pH 5.0. For fluoride binding to CCP, the binding constant increased from 8.41(+\/- 0.54) x 10(3) M-1 at pH 7.0 to 3.11(+\/- 0.09) x 10(5) M-1 at pH 5.0 and the binding enthalpy increased from -71.9 +\/- 1.1 kJ.mol-1 at pH 7.0 to -67.0 +\/- 1.9 kJ.mol-1 at pH 5.0. The binding enthalpies for D82CCP were about the same as those for CCP. However, the binding constants for cyanide and fluoride to D82CCP were respectively a factor of two less and at least an order of magnitude less than the corresponding binding constants of CCP. Decreased ligand-binding strength in the D82CCP mutant is thus entirely due to entropic effects.<\/jats:p>","DOI":"10.1042\/bj3020437","type":"journal-article","created":{"date-parts":[[2015,8,10]],"date-time":"2015-08-10T21:39:10Z","timestamp":1439242750000},"page":"437-442","update-policy":"http:\/\/dx.doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Thermodynamics of hydrogen cyanide and hydrogen fluoride binding to cytochrome <i>c<\/i> peroxidase and its Asn-82\u2192Asp mutant"],"prefix":"10.1042","volume":"302","author":[{"given":"S F","family":"DeLauder","sequence":"first","affiliation":[{"name":"Center for Advanced Research in Biotechnology, National Institute of Standards and Technology, 9600 Gudelsky Drive, Rockville, MD 20850, U.S.A."},{"name":"Department of Chemistry, Howard University, Washington DC 20059, U.S.A."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J M","family":"Mauro","sequence":"additional","affiliation":[{"name":"Center for Advanced Research in Biotechnology, National Institute of Standards and Technology, 9600 Gudelsky Drive, Rockville, MD 20850, U.S.A."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T L","family":"Poulos","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology & Biochemistry, University of California, Irving, CA 92717, U.S.A."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J C","family":"Williams","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Howard University, Washington DC 20059, U.S.A."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F P","family":"Schwarz","sequence":"additional","affiliation":[{"name":"Center for Advanced Research in Biotechnology, National Institute of Standards and Technology, 9600 Gudelsky Drive, Rockville, MD 20850, U.S.A."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","container-title":["Biochemical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/302\/2\/437\/614556\/bj3020437.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/302\/2\/437\/614556\/bj3020437.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,23]],"date-time":"2021-11-23T23:09:46Z","timestamp":1637708986000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/biochemj\/article\/302\/2\/437\/31048\/Thermodynamics-of-hydrogen-cyanide-and-hydrogen"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1994,9,1]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1994,9,1]]}},"URL":"https:\/\/doi.org\/10.1042\/bj3020437","relation":{},"ISSN":["0264-6021","1470-8728"],"issn-type":[{"value":"0264-6021","type":"print"},{"value":"1470-8728","type":"electronic"}],"subject":[],"published":{"date-parts":[[1994,9,1]]}}}