{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,7,28]],"date-time":"2024-07-28T15:19:48Z","timestamp":1722179988611},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"1","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1994,10,1]]},"abstract":"<jats:p>Seven monoclonal antibodies (mAbs) were developed against neurotoxin I (NT-1), a protein from central Asian cobra (Naja naja oxiana) venom which binds specifically to nicotinic acetylcholine receptor (AchR). All of the mAbs cross-reacted with another long-chain post-synaptic neurotoxin, Bungarus multicinctus alpha-bungarotoxin (alpha-BT), but not Naja naja kaouthia alpha-cobratoxin, in an enzyme-linked immunosorbent assay (e.l.i.s.a.). Short-chain post-synaptic neurotoxins like Naja naja atra cobrotoxin, Laticauda semifasciata erabutoxin b, or N. n. oxiana neurotoxin II did not cross-react with the NT-1 mAbs, but an antigen(s) found in Dendroaspis polylepis, Acanthophis antarcticus and Pseudechis australis venoms was immunoreactive. The e.l.i.s.a. readings for dithiothreitol-reduced NT-1 and NT-1 mAbs ranged from 13 to 27% of those for native toxin but reduced alpha-BT was not immunoreactive. Synthetic NT-1 peptides were used in epitope-mapping studies and two, non-contiguous regions (Cys15-Tyr23 and Lys25-Gly33 or Pro17-Lys25 and Asp29-Lys37) were recognized by the NT-1 mAbs. The NT-1 mAbs individually inhibited 31-71% of alpha-BT binding to AchR in vitro and afforded a slight protective effect in vivo with a toxin: antibody mole ratio of 1:1.5. This report is the first to describe mAbs which recognize and protect against a heterologous, long-chain, post-synaptic neurotoxin from snake venom.<\/jats:p>","DOI":"10.1042\/bj3030163","type":"journal-article","created":{"date-parts":[[2015,8,10]],"date-time":"2015-08-10T21:40:01Z","timestamp":1439242801000},"page":"163-170","update-policy":"http:\/\/dx.doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Characterization of monoclonal antibodies against <i>Naja naja oxiana<\/i> neurotoxin I"],"prefix":"10.1042","volume":"303","author":[{"given":"B G","family":"Stiles","sequence":"first","affiliation":[{"name":"Toxinology Division, United States Army Medical Research Institute of Infectious Diseases, Fort Detrick, Frederick, MD 21702-5011, U.S.A."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F W","family":"Sexton","sequence":"additional","affiliation":[{"name":"Toxinology Division, United States Army Medical Research Institute of Infectious Diseases, Fort Detrick, Frederick, MD 21702-5011, U.S.A."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S B","family":"Guest","sequence":"additional","affiliation":[{"name":"Virology, United States Army Medical Research Institute of Infectious Diseases, Fort Detrick, Frederick, MD 21702-5011, U.S.A."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M A","family":"Olson","sequence":"additional","affiliation":[{"name":"Toxinology Division, United States Army Medical Research Institute of Infectious Diseases, Fort Detrick, Frederick, MD 21702-5011, U.S.A."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D C","family":"Hack","sequence":"additional","affiliation":[{"name":"Medical Divisions, United States Army Medical Research Institute of Infectious Diseases, Fort Detrick, Frederick, MD 21702-5011, U.S.A."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","container-title":["Biochemical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/303\/1\/163\/615019\/bj3030163.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/303\/1\/163\/615019\/bj3030163.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,23]],"date-time":"2021-11-23T23:14:32Z","timestamp":1637709272000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/biochemj\/article\/303\/1\/163\/31259\/Characterization-of-monoclonal-antibodies-against"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1994,10,1]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1994,10,1]]}},"URL":"https:\/\/doi.org\/10.1042\/bj3030163","relation":{},"ISSN":["0264-6021","1470-8728"],"issn-type":[{"value":"0264-6021","type":"print"},{"value":"1470-8728","type":"electronic"}],"subject":[],"published":{"date-parts":[[1994,10,1]]}}}