{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T23:58:20Z","timestamp":1781135900155,"version":"3.54.1"},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"1","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1994,10,1]]},"abstract":"<jats:p>Treatment of the cultured human breast-cancer cells BC-M1 with dexamethasone induced a placental-type alkaline phosphatase (ALP). Both the ALP activity and the mRNA level in the cells were increased. The induction of ALP activity by dexamethasone was time- and dose-dependent. The accumulation of ALP mRNA was inhibited by both actinomycin D and cycloheximide, indicating that its induction is a complex event and may involve other regulatory proteins. Retinoic acid showed opposing effects with dexamethasone on the expression of alkaline phosphatase. Retinoic acid (RA) and phorbol 12-myristate 13-acetate also substantially reduced the dexamethasone-induced expression of ALP. Studies on thermostability and sensitivity to various amino acid inhibitors indicated that the BC-M1 ALP is most similar to the placental form. Northern hybridization analysis also revealed that ALP mRNA transcripts in BC-M1 and term placenta are similar in size and are distinct from that of the placental-like mRNA transcript in choriocarcinoma cells. Analysis of the degradation of BC-M1 ALP mRNA showed a similar half-life of 27 h in the untreated and in dexamethasone- or RA-treated cells. These findings demonstrated that the induction of ALP in BC-M1 cells by dexamethasone is mainly due to the increase in the transcription of the ALP gene.<\/jats:p>","DOI":"10.1042\/bj3030199","type":"journal-article","created":{"date-parts":[[2015,8,10]],"date-time":"2015-08-10T21:40:01Z","timestamp":1439242801000},"page":"199-205","update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":27,"title":["Regulation of the expression of alkaline phosphatase in a human breast-cancer cell line"],"prefix":"10.1042","volume":"303","author":[{"given":"T C","family":"Chang","sequence":"first","affiliation":[{"name":"Department of Biochemistry, National Defense Medical Center, Taipei, Taiwan, Republic of China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J K","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, National Defense Medical Center, Taipei, Taiwan, Republic of China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M W","family":"Hung","sequence":"additional","affiliation":[{"name":"Department of Medical Research, Veterans General Hospital, Taipei, Taiwan, Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C H","family":"Chiao","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, National Defense Medical Center, Taipei, Taiwan, Republic of China."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L C","family":"Tsai","sequence":"additional","affiliation":[{"name":"Department of Medical Research, Veterans General Hospital, Taipei, Taiwan, Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G G","family":"Chang","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, National Defense Medical Center, Taipei, Taiwan, Republic of China."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"288","container-title":["Biochemical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/303\/1\/199\/614824\/bj3030199.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/303\/1\/199\/614824\/bj3030199.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,23]],"date-time":"2021-11-23T23:12:51Z","timestamp":1637709171000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/biochemj\/article\/303\/1\/199\/31169\/Regulation-of-the-expression-of-alkaline"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1994,10,1]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1994,10,1]]}},"URL":"https:\/\/doi.org\/10.1042\/bj3030199","relation":{},"ISSN":["0264-6021","1470-8728"],"issn-type":[{"value":"0264-6021","type":"print"},{"value":"1470-8728","type":"electronic"}],"subject":[],"published":{"date-parts":[[1994,10,1]]}}}