{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T15:19:40Z","timestamp":1761059980664},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"2","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1994,12,1]]},"abstract":"<jats:p>By contrast with mammalian beta-adrenergic receptors, the avian isoform elicits two distinct effector responses, activation of adenylate cyclase and polyphosphoinositide-specific phospholipase C (PLC) leading to the accumulation of both cyclic adenosine monophosphate (cyclic AMP) and inositol phosphates. We have investigated the mechanisms of beta-adrenergic receptor signalling in turkey erythrocytes. Stimulation of adenylate cyclase by the beta-adrenergic-receptor agonist isoprenaline exhibits a 30-fold lower EC50 than that for PLC activation, which may indicate a marked receptor reserve for the former effector. Similar Ki values were obtained for the inhibition of both responses by four beta-adrenergic antagonists, arguing that a single receptor population is responsible for both effects. Antibodies raised against G-protein peptide sequences were used to show that the identity of the G-protein mediating the PLC response was an avian homologue of G11, the level of expression of which was very similar to that of the stimulatory G-protein of adenylate cyclase, Gs. Thus a single population of beta-adrenergic receptors apparently interacts with distinct G-proteins to activate different effectors. The stoichiometries of the receptor-G-protein-effector interactions are therefore similar for both second-messenger responses and the data are discussed in terms of the different efficacies observed for each response.<\/jats:p>","DOI":"10.1042\/bj3040359","type":"journal-article","created":{"date-parts":[[2015,8,10]],"date-time":"2015-08-10T21:40:39Z","timestamp":1439242839000},"page":"359-364","update-policy":"http:\/\/dx.doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["The turkey erythrocyte <i>\u03b2<\/i>-adrenergic receptor couples to both adenylate cyclase and phospholipase C via distinct G-protein \u03b1 subunits"],"prefix":"10.1042","volume":"304","author":[{"given":"S R","family":"James","sequence":"first","affiliation":[{"name":"Department of Biochemistry, Medical Sciences Institute, University of Dundee, Dundee DD1 4HN, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C","family":"Vaziri","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Medical Sciences Institute, University of Dundee, Dundee DD1 4HN, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T R","family":"Walker","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Medical Sciences Institute, University of Dundee, Dundee DD1 4HN, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G","family":"Milligan","sequence":"additional","affiliation":[{"name":"Molecular Pharmacology Group, Departments of Biochemistry and Pharmacology, University of Glasgow, Glasgow G1 2 8QQ, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C P","family":"Downes","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Medical Sciences Institute, University of Dundee, Dundee DD1 4HN, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","container-title":["Biochemical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/304\/2\/359\/725579\/bj3040359.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/304\/2\/359\/725579\/bj3040359.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,23]],"date-time":"2021-11-23T23:20:11Z","timestamp":1637709611000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/biochemj\/article\/304\/2\/359\/31566\/The-turkey-erythrocyte-adrenergic-receptor-couples"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1994,12,1]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1994,12,1]]}},"URL":"https:\/\/doi.org\/10.1042\/bj3040359","relation":{},"ISSN":["0264-6021","1470-8728"],"issn-type":[{"value":"0264-6021","type":"print"},{"value":"1470-8728","type":"electronic"}],"subject":[],"published":{"date-parts":[[1994,12,1]]}}}