{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,4]],"date-time":"2025-09-04T14:13:16Z","timestamp":1756995196846},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"3","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1994,12,15]]},"abstract":"<jats:p>The expression of purinergic receptors on human T-cells was investigated and the receptors were shown to be functionally coupled to intracellular signals in two out of eight T-leukaemia cell-lines. Addition of adenine nucleotides resulted in mobilization of intracellular Ca2+ in HPB-ALL cells and a cell line (CB1) recently isolated from a patient with T-acute lymphoblastic leukaemia. Of a range of nucleotides tested only ADP and ATP elevated intracellular levels of Ca2+, with ADP being the more potent agonist. Ca2+ mobilization by ATP was accompanied by increased inositol phosphate production and was blocked by the purinergic receptor antagonist, Reactive Blue 2, indicating that ATP was interacting with a P2y receptor. Intracellular Ca2+ release triggered by ADP was independent of both inositol phosphate production and protein tyrosine phosphorylation. Expression of the transmembrane phosphotyrosine phosphatase, CD45, had no effect on ADP-stimulated Ca2+ mobilization. Our results show that functional P2y receptors can be expressed on T-cells, and also identify a novel T-cell ADP receptor. Signals mediated by these purinergic receptors could play important roles in modulating T-cell function.<\/jats:p>","DOI":"10.1042\/bj3040769","type":"journal-article","created":{"date-parts":[[2015,8,10]],"date-time":"2015-08-10T21:40:39Z","timestamp":1439242839000},"page":"769-774","update-policy":"http:\/\/dx.doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Mobilization of intracellular Ca2+ by adenine nucleotides in human T-leukaemia cells: evidence for ADP-specific and P2y-purinergic receptors"],"prefix":"10.1042","volume":"304","author":[{"given":"M","family":"Biffen","sequence":"first","affiliation":[{"name":"T-Cell Laboratory, Department of Immunology, Babraham Institute, Babraham, Cambridge CB2 4AT, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D R","family":"Alexander","sequence":"additional","affiliation":[{"name":"T-Cell Laboratory, Department of Immunology, Babraham Institute, Babraham, Cambridge CB2 4AT, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","container-title":["Biochemical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/304\/3\/769\/792997\/bj3040769.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/304\/3\/769\/792997\/bj3040769.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,23]],"date-time":"2021-11-23T23:29:02Z","timestamp":1637710142000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/biochemj\/article\/304\/3\/769\/31761\/Mobilization-of-intracellular-Ca2-by-adenine"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1994,12,15]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1994,12,15]]}},"URL":"https:\/\/doi.org\/10.1042\/bj3040769","relation":{},"ISSN":["0264-6021","1470-8728"],"issn-type":[{"value":"0264-6021","type":"print"},{"value":"1470-8728","type":"electronic"}],"subject":[],"published":{"date-parts":[[1994,12,15]]}}}