{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T11:10:08Z","timestamp":1770721808674,"version":"3.49.0"},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"1","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1995,4,1]]},"abstract":"<jats:p>Ligation of membrane immunoglobulin M (mIgM) receptor in the Ramos B-cell line induced tyrosine phosphorylation of several intracellular substrates, including the adaptor protein. Shc. Phosphorylated Shc could be seen to associate with Grb2 in a complex which included hSOS. Inasmuch as hSOS is involved in p21ras activation, we also demonstrated that mIgM ligation activated a Ras-dependent kinase cascade in which sequential activation of Raf-1 and MEK-1 culminates in the activation of p42 mitogen-activated protein (MAP) kinase (ERK-2). The tumour promoter and protein kinase C agonist, phorbol 12-myristate 13-acetate (PMA), also activated Raf-1, MEK-1, and MAP kinase in Ramos cells, but did not induce tyrosine phosphorylation of Shc or Shc\/Grb2 association. Okadaic acid, another tumour promoter and serine\/threonine phosphatase inhibitor, activated p42 MAP kinase without activating Raf-1 or MEK-1, suggesting the existence of a serine\/threonine phosphatase which directly regulates MAP kinase activity.<\/jats:p>","DOI":"10.1042\/bj3070215","type":"journal-article","created":{"date-parts":[[2015,8,10]],"date-time":"2015-08-10T21:42:27Z","timestamp":1439242947000},"page":"215-223","update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["The membrane immunoglobulin receptor utilizes a Shc\/Grb2\/hSOS complex for activation of the mitogen-activated protein kinase cascade in a B-cell line"],"prefix":"10.1042","volume":"307","author":[{"given":"G","family":"Kumar","sequence":"first","affiliation":[{"name":"Division of Clinical Immunology and Allergy, Department of Medicine, UCLA School of Medicine, 10833 LeConte Avenue, Los Angeles, CA 90024, U.S.A."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S","family":"Wang","sequence":"additional","affiliation":[{"name":"Division of Clinical Immunology and Allergy, Department of Medicine, UCLA School of Medicine, 10833 LeConte Avenue, Los Angeles, CA 90024, U.S.A."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S","family":"Gupta","sequence":"additional","affiliation":[{"name":"Division of Clinical Immunology and Allergy, Department of Medicine, UCLA School of Medicine, 10833 LeConte Avenue, Los Angeles, CA 90024, U.S.A."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A","family":"Nel","sequence":"additional","affiliation":[{"name":"Division of Clinical Immunology and Allergy, Department of Medicine, UCLA School of Medicine, 10833 LeConte Avenue, Los Angeles, CA 90024, U.S.A."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","container-title":["Biochemical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/307\/1\/215\/615948\/bj3070215.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/307\/1\/215\/615948\/bj3070215.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,23]],"date-time":"2021-11-23T23:21:55Z","timestamp":1637709715000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/biochemj\/article\/307\/1\/215\/31617\/The-membrane-immunoglobulin-receptor-utilizes-a"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1995,4,1]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1995,4,1]]}},"URL":"https:\/\/doi.org\/10.1042\/bj3070215","relation":{},"ISSN":["0264-6021","1470-8728"],"issn-type":[{"value":"0264-6021","type":"print"},{"value":"1470-8728","type":"electronic"}],"subject":[],"published":{"date-parts":[[1995,4,1]]}}}