{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,6]],"date-time":"2026-03-06T06:12:17Z","timestamp":1772777537440,"version":"3.50.1"},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"3","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1995,11,1]]},"abstract":"<jats:p>Using gel-filtration chromatography and reverse-phase (RP) HPLC we have purified a presynaptic neurotoxin (designated as trimucrotoxin) from the crude venom of Taiwan habu (Trimeresurus mucrosquamatus). Its complete primary structure was solved by an automated N-terminal sequencing and cDNA sequencing method. The enzyme inhibited the twitch of the chick biventer cervicis muscle at 0.1-1 micrograms\/ml and showed lethality in mice (LD50 = 1.2 micrograms\/g, when given intravenously). Trimucrotoxin exists mainly as a homodimer of 14 kDa subunits as shown by a gel-filtration experiment, and dissociates into monomers during SDS\/PAGE in the absence of Ca2+. However, most of trimucrotoxin migrated as slowly as a trimer during nondenaturing SDS\/PAGE in the presence of Ca2+ or Sr2+. Its amino acid sequence identity to crotoxin B and agkistrodotoxin is about 75%, and its cDNA sequence is 82% identical to that of crotoxin B. Rabbit antiserum against trimucrotoxin also cross-reacted with the other crotalid neurotoxic phospholipases A2. Furthermore, the purified acidic subunit of crotoxin potentiated the neurotoxicity of trimucrotoxin. A comparison of the sequences of these crotalid neurotoxins revealed some common features of the possible neurotoxic sites, including residues 6, 11, 76-81 and 119-125.<\/jats:p>","DOI":"10.1042\/bj3110895","type":"journal-article","created":{"date-parts":[[2015,8,10]],"date-time":"2015-08-10T21:45:43Z","timestamp":1439243143000},"page":"895-900","update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":56,"title":["Molecular cloning and characterization of a neurotoxic phospholipase A2 from the venom of Taiwan habu (<i>Trimeresurus mucrosquamatus<\/i>)"],"prefix":"10.1042","volume":"311","author":[{"given":"I H","family":"Tsai","sequence":"first","affiliation":[{"name":"Institute of Biological Chemistry, Academia Sinica, Taipei, Taiwan, Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P J","family":"Lu","sequence":"additional","affiliation":[{"name":"Institute of Biological Chemistry, Academia Sinica, Taipei, Taiwan, Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Y M","family":"Wang","sequence":"additional","affiliation":[{"name":"Institute of Biological Chemistry, Academia Sinica, Taipei, Taiwan, Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C L","family":"Ho","sequence":"additional","affiliation":[{"name":"Institute of Biological Chemistry, Academia Sinica, Taipei, Taiwan, Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L L","family":"Liaw","sequence":"additional","affiliation":[{"name":"Institute of Biological Chemistry, Academia Sinica, Taipei, Taiwan, Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","container-title":["Biochemical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/311\/3\/895\/617947\/bj3110895.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/311\/3\/895\/617947\/bj3110895.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,23]],"date-time":"2021-11-23T23:53:34Z","timestamp":1637711614000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/biochemj\/article\/311\/3\/895\/32380\/Molecular-cloning-and-characterization-of-a"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1995,11,1]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1995,11,1]]}},"URL":"https:\/\/doi.org\/10.1042\/bj3110895","relation":{},"ISSN":["0264-6021","1470-8728"],"issn-type":[{"value":"0264-6021","type":"print"},{"value":"1470-8728","type":"electronic"}],"subject":[],"published":{"date-parts":[[1995,11,1]]}}}