{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,24]],"date-time":"2026-07-24T10:14:09Z","timestamp":1784888049350,"version":"3.55.0"},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"2","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1996,1,15]]},"abstract":"<jats:p>Little is known about whether the properties of respiratory mucins are altered as a result of airway irritation, but histochemical studies of respiratory tract secretory cells show a more \u2018acidic\u2019 staining pattern after exposure to tobacco smoke. Furthermore it has been suggested that proteoglycans are the major glycoconjugates in \u2018normal\u2019 respiratory secretions, whereas mucins predominate in sputum. To investigate these observations further, mucins from secretions collected from the tracheal surface of healthy non-smoking \u2018normal\u2019 subjects and sputum from patients with chronic bronchitis were compared. All samples contained one major mucin population after density-gradient centrifugation, and a small amount of \u2018denser\u2019 mucin was present in some chronic bronchitic and one of the \u2018normal\u2019 samples. Proteoglycans were not a major component of \u2018normal\u2019 secretions. The major mucin population from chronic bronchitic samples had molecular masses between 10 and 30 MDa and behaved as random coils in solution. Whole mucins from \u2018normal\u2019 individuals and chronic bronchitic patients were excluded from Sepharose CL-2B, whereas reduced subunits were included. Proteolysis of subunits yielded two populations of high-molecular-mass glycopeptides differing in size, suggesting the presence of two different tandem repeat regions in the mucins. Finally, mucins from patients with chronic bronchitis are less, rather than more, acidic than those from \u2018normal\u2019 individuals. Mucins from bronchitic sputum and \u2018normal\u2019 secretions are thus similar in their macromolecular properties, but differ slightly in charge density.<\/jats:p>","DOI":"10.1042\/bj3130431","type":"journal-article","created":{"date-parts":[[2015,8,10]],"date-time":"2015-08-10T21:47:00Z","timestamp":1439243220000},"page":"431-439","update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":44,"title":["Mucins in airway secretions from healthy and chronic bronchitic subjects"],"prefix":"10.1042","volume":"313","author":[{"given":"Julia R.","family":"DAVIES","sequence":"first","affiliation":[{"name":"Department of Cell and Molecular Biology, Section for Molecular Pathogenesis, Lund University, Box 94, S-221 00, Lund, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hans W.","family":"HOVENBERG","sequence":"additional","affiliation":[{"name":"Department of Cell and Molecular Biology, Section for Molecular Pathogenesis, Lund University, Box 94, S-221 00, Lund, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carl-Johan","family":"LIND\u00c9N","sequence":"additional","affiliation":[{"name":"Department of Lung Medicine, University Hospital of Lund, S-221 85, Lund, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard","family":"HOWARD","sequence":"additional","affiliation":[{"name":"Department of Anaesthetics, St George's Hospital, London SW17 0RE, U.K."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul S.","family":"RICHARDSON","sequence":"additional","affiliation":[{"name":"Department of Physiology, St George's Hospital Medical School, London SW17 0RE, U.K."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John K.","family":"SHEEHAN","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, University of Manchester, Manchester M13 9PT, U.K."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ingemar","family":"CARLSTEDT","sequence":"additional","affiliation":[{"name":"Department of Cell and Molecular Biology, Section for Molecular Pathogenesis, Lund University, Box 94, S-221 00, Lund, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"288","container-title":["Biochemical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/313\/2\/431\/618244\/bj3130431.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/313\/2\/431\/618244\/bj3130431.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,23]],"date-time":"2021-11-23T23:56:22Z","timestamp":1637711782000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/biochemj\/article\/313\/2\/431\/32453\/Mucins-in-airway-secretions-from-healthy-and"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1996,1,15]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1996,1,15]]}},"URL":"https:\/\/doi.org\/10.1042\/bj3130431","relation":{},"ISSN":["0264-6021","1470-8728"],"issn-type":[{"value":"0264-6021","type":"print"},{"value":"1470-8728","type":"electronic"}],"subject":[],"published":{"date-parts":[[1996,1,15]]}}}