{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,28]],"date-time":"2026-02-28T07:34:27Z","timestamp":1772264067746,"version":"3.50.1"},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"3","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1996,2,1]]},"abstract":"<jats:p>Seven toxic peptides from the venom of Tityus bahiensis and Tityus stigmurus were isolated and sequenced, five of them to completion. The most abundant peptide from each of these two species of scorpion was 95% identical with that of toxin \u03b3 from the venom of Tityus serrulatus. They were consequently named \u03b3-b and \u03b3-st respectively. The genes encoding these new \u03b3-like peptides were cloned and sequenced by utilizing oligonucleotides synthesized according to known cDNA sequences of toxin \u03b3, and amplified by PCR on templates of DNA purified from both T. bahiensis and T. stigmurus. They contain an intron of approx. 470 bp. Possible mechanisms of processing and expressing these peptides are discussed, in view of the fact that glycine is the first residue of the N-terminal sequence of T. stigmurus, whereas lysine is the residue at position 1 of toxin \u03b3 from T. serrulatus and T. bahiensis. In addition, chemical characterization of the less abundant toxic peptides showed the presence of at least four distinct families of peptides in all three species of the genus Tityus studied. There is a large degree of similarity among peptides from different venoms of the same family. By using specific horse and rabbit antisera, the venoms of T. bahiensis, T. serrulatus and T. stigmurus were compared. They showed an extended degree of cross-reactivity. Thus these three species of scorpion have similar toxic components, the genes of which are similarly organized, processed and expressed.<\/jats:p>","DOI":"10.1042\/bj3130753","type":"journal-article","created":{"date-parts":[[2015,8,10]],"date-time":"2015-08-10T21:47:00Z","timestamp":1439243220000},"page":"753-760","update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":70,"title":["Toxic peptides and genes encoding toxin <i>\u03b3<\/i> of the Brazilian scorpions <i>Tityus bahiensis<\/i> and <i>Tityus stigmurus<\/i>"],"prefix":"10.1042","volume":"313","author":[{"given":"Baltazar","family":"BECERRIL","sequence":"first","affiliation":[{"name":"Department of Molecular Recognition and Structural Biology, Instituto de Biotecnolog\u00eda, Universidad Nacional Aut\u00f3noma de Mexico, Avenida Universidad, 2001, Apartado Postal 510-3, Cuernavaca-62271, M\u00e9xico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Miguel","family":"CORONA","sequence":"additional","affiliation":[{"name":"Department of Molecular Recognition and Structural Biology, Instituto de Biotecnolog\u00eda, Universidad Nacional Aut\u00f3noma de Mexico, Avenida Universidad, 2001, Apartado Postal 510-3, Cuernavaca-62271, M\u00e9xico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fredy I. V.","family":"CORONAS","sequence":"additional","affiliation":[{"name":"Department of Molecular Recognition and Structural Biology, Instituto de Biotecnolog\u00eda, Universidad Nacional Aut\u00f3noma de Mexico, Avenida Universidad, 2001, Apartado Postal 510-3, Cuernavaca-62271, M\u00e9xico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fernando","family":"ZAMUDIO","sequence":"additional","affiliation":[{"name":"Department of Molecular Recognition and Structural Biology, Instituto de Biotecnolog\u00eda, Universidad Nacional Aut\u00f3noma de Mexico, Avenida Universidad, 2001, Apartado Postal 510-3, Cuernavaca-62271, M\u00e9xico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Emma S.","family":"CALDERONARANDA","sequence":"additional","affiliation":[{"name":"Department of Molecular Recognition and Structural Biology, Instituto de Biotecnolog\u00eda, Universidad Nacional Aut\u00f3noma de Mexico, Avenida Universidad, 2001, Apartado Postal 510-3, Cuernavaca-62271, M\u00e9xico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"suffix":"Jr.","given":"Paul L.","family":"FLETCHER","sequence":"additional","affiliation":[{"name":"Centro de Investigaci\u00f3n en Biotechnolog\u00eda, Universidad Aut\u00f3noma de Estado de Morelos, Avenida Universidad, 1001-62210 Cuernavaca, M\u00e9xico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Brian M.","family":"MARTIN","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, School of Medicine, East Carolina University, Greenville, NC 27858, U.S.A."},{"name":"Unit on Molecular Structures, Clinical Neuroscience Branch, National Institute of Mental Health, Bethesda, MD 20892, U.S.A."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lourival D.","family":"POSSANI","sequence":"additional","affiliation":[{"name":"Unit on Molecular Structures, Clinical Neuroscience Branch, National Institute of Mental Health, Bethesda, MD 20892, U.S.A."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","container-title":["Biochemical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/313\/3\/753\/617413\/bj3130753.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/313\/3\/753\/617413\/bj3130753.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,23]],"date-time":"2021-11-23T23:49:34Z","timestamp":1637711374000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/biochemj\/article\/313\/3\/753\/32267\/Toxic-peptides-and-genes-encoding-toxin-of-the"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1996,2,1]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1996,2,1]]}},"URL":"https:\/\/doi.org\/10.1042\/bj3130753","relation":{},"ISSN":["0264-6021","1470-8728"],"issn-type":[{"value":"0264-6021","type":"print"},{"value":"1470-8728","type":"electronic"}],"subject":[],"published":{"date-parts":[[1996,2,1]]}}}