{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,31]],"date-time":"2023-10-31T05:05:17Z","timestamp":1698728717393},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"1","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1996,2,15]]},"abstract":"<jats:p>We have previously described the abnormal localization of resident Golgi proteins and O-glycans in the rough endoplasmic reticulum of mucin-secreting HT-29 M6 colon cancer cells, suggesting altered protein trafficking in these cells [Egea, Franci~A, Gamb\u00fas, Lesuffleur, Zweibaum and Real (1993) J. Cell Sci. 105, 819\u2013830]. In the present work, we have chosen lysosomal \u03b1-glucosidase as a reporter to examine the intracellular traffic of glycoproteins in M6 cells. We have compared the synthesis and processing of \u03b1-glucosidase in mucin-secreting M6 cells and in Caco-2 colon cancer cells, the latter resembling normal absorptive intestinal epithelium. Our results show that \u03b1-glucosidase processing and secretion is markedly delayed in M6 cells as compared with Caco-2 cells or normal fibroblasts, and this delay is caused by an accumulation of \u03b1-glucosidase precursor form in the trans-Golgi network. Furthermore, treatment of Caco-2 cells with brefeldin A led to changes in \u03b1-glucosidase maturation similar to those observed in untreated M6 cells. To determine whether altered processing occurs in other cultured cells, a panel of cancer cell lines and cultures from normal exocrine pancreas were examined. In pancreas-derived cultures, \u03b1-glucosidase showed a processing pattern different from that described until now. Only HT-29 cells and HT-29-derived subpopulations displayed a defect in \u03b1-glucosidase maturation. In conclusion, \u03b1-glucosidase processing is more diverse than has previously been described; this finding may have tissue-specific functional implications.<\/jats:p>","DOI":"10.1042\/bj3140033","type":"journal-article","created":{"date-parts":[[2015,8,10]],"date-time":"2015-08-10T21:47:57Z","timestamp":1439243277000},"page":"33-40","update-policy":"http:\/\/dx.doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Lysosomal \u03b1-glucosidase: cell-specific processing and altered maturation in HT-29 colon cancer cells"],"prefix":"10.1042","volume":"314","author":[{"given":"Clara","family":"FRANC\u00cd","sequence":"first","affiliation":[{"name":"Departament d'Immunologia, Institut Municipal d'Investigaci\u00f3 M\u00e8dica, Universitat Aut\u00f2noma de Barcelona, 08003-Barcelona, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gustavo","family":"EGEA","sequence":"additional","affiliation":[{"name":"Departament de Biologia Cel.lular, Facultat de Medicina, Universitat de Barcelona, 08036-Barcelona, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rosa","family":"ARRIBAS","sequence":"additional","affiliation":[{"name":"Departament d'Immunologia, Institut Municipal d'Investigaci\u00f3 M\u00e8dica, Universitat Aut\u00f2noma de Barcelona, 08003-Barcelona, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Arnold J. J.","family":"REUSER","sequence":"additional","affiliation":[{"name":"Department of Clinical Genetics, Erasmus University, P.O. Box 1738, 3000 DR Rotterdam, The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Francisco X.","family":"REAL","sequence":"additional","affiliation":[{"name":"Departament d'Immunologia, Institut Municipal d'Investigaci\u00f3 M\u00e8dica, Universitat Aut\u00f2noma de Barcelona, 08003-Barcelona, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","container-title":["Biochemical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/314\/1\/33\/618568\/bj3140033.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/314\/1\/33\/618568\/bj3140033.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,23]],"date-time":"2021-11-23T23:24:22Z","timestamp":1637709862000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/biochemj\/article\/314\/1\/33\/31654\/Lysosomal-glucosidase-cell-specific-processing-and"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1996,2,15]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1996,2,15]]}},"URL":"https:\/\/doi.org\/10.1042\/bj3140033","relation":{},"ISSN":["0264-6021","1470-8728"],"issn-type":[{"value":"0264-6021","type":"print"},{"value":"1470-8728","type":"electronic"}],"subject":[],"published":{"date-parts":[[1996,2,15]]}}}