{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T04:55:31Z","timestamp":1791089731559,"version":"4.1.0"},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"1","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1996,2,15]]},"abstract":"<jats:p>Optical spectroscopy and reverse-phase HPLC were used to investigate the binding of Hg(II) to plant metal-binding peptides (phytochelatins) with the structure (\u03b3Glu-Cys)2Gly, (\u03b3Glu-Cys)3Gly and (\u03b3Glu-Cys)4Gly. Glutathione-mediated transfer of Hg(II) into phytochelatins and the transfer of the metal ion from one phytochelatin to another was also studied using reverse-phase HPLC. The saturation of Hg(II)-induced bands in the UV\/visible and CD spectra of (\u03b3Glu-Cys)2Gly suggested the formation of a single Hg(II)-binding species of this peptide with a stoichiometry of one metal ion per peptide molecule. The separation of apo-(\u03b3Glu-Cys)2Gly from its Hg(II) derivative on a C18 reverse-phase column also indicated the same metal-binding stoichiometry. The UV\/visible spectra of both (\u03b3Glu-Cys)3Gly and (\u03b3Glu-Cys)4Gly at pH 7.4 showed distinct shoulders in the ligand-to-metal charge-transfer region at 280\u2013290 nm. Two distinct Hg(II)-binding species, occurring at metal-binding stoichiometries of around 1.25 and 2.0 Hg(II) ions per peptide molecule, were observed for (\u03b3Glu-Cys)3Gly. These species exhibited specific spectral features in the charge-transfer region and were separable by HPLC. Similarly, two main Hg(II)-binding species of (\u03b3Glu-Cys)4Gly were observed by UV\/visible and CD spectroscopy at metal-binding stoichiometries of around 1.25 and 2.5 respectively. Only a single peak of Hg(II)\u2013(\u03b3Glu-Cys)4Gly complexes was resolved under the conditions used for HPLC. The overall Hg(II)-binding stoichiometries of phytochelatins were similar at pH 2.0 and at pH 7.4, indicating that pH did not influence the final Hg(II)-binding capacity of these peptides. The reverse-phase HPLC assays indicated a rapid transfer of Hg(II) from glutathione to phytochelatins. These assays also demonstrated a facile transfer of the metal ion from shorter- to longer-chain phytochelatins. The strength of Hg(II) binding to glutathione and phytochelatins followed the order: \u03b3Glu-Cys-Gly &amp;lt; (\u03b3Glu-Cys)2Gly &amp;lt; (\u03b3Glu-Cys)3Gly &amp;lt; (\u03b3Glu-Cys)4Gly.<\/jats:p>","DOI":"10.1042\/bj3140073","type":"journal-article","created":{"date-parts":[[2015,8,10]],"date-time":"2015-08-10T21:47:57Z","timestamp":1439243277000},"page":"73-82","update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":68,"title":["Optical spectroscopic and reverse-phase HPLC analyses of Hg(II) binding to phytochelatins"],"prefix":"10.1042","volume":"314","author":[{"given":"Rajesh K.","family":"MEHRA","sequence":"first","affiliation":[{"name":"Environmental Toxicology Graduate Program, Entomology Department, University of California, Riverside, CA 92521, U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jose","family":"MICLAT","sequence":"additional","affiliation":[{"name":"Environmental Toxicology Graduate Program, Entomology Department, University of California, Riverside, CA 92521, U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"V. Ramana","family":"KODATI","sequence":"additional","affiliation":[{"name":"Environmental Toxicology Graduate Program, Entomology Department, University of California, Riverside, CA 92521, U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rizwana","family":"ABDULLAH","sequence":"additional","affiliation":[{"name":"Environmental Toxicology Graduate Program, Entomology Department, University of California, Riverside, CA 92521, U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas C.","family":"HUNTER","sequence":"additional","affiliation":[{"name":"Environmental Toxicology Graduate Program, Entomology Department, University of California, Riverside, CA 92521, U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Priti","family":"MULCHANDANI","sequence":"additional","affiliation":[{"name":"Environmental Toxicology Graduate Program, Entomology Department, University of California, Riverside, CA 92521, U.S.A."}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"288","container-title":["Biochemical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/314\/1\/73\/618604\/bj3140073.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/314\/1\/73\/618604\/bj3140073.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,23]],"date-time":"2021-11-23T23:25:08Z","timestamp":1637709908000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/biochemj\/article\/314\/1\/73\/31668\/Optical-spectroscopic-and-reverse-phase-HPLC"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1996,2,15]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1996,2,15]]}},"URL":"https:\/\/doi.org\/10.1042\/bj3140073","relation":{},"ISSN":["0264-6021","1470-8728"],"issn-type":[{"value":"0264-6021","type":"print"},{"value":"1470-8728","type":"electronic"}],"subject":[],"published":{"date-parts":[[1996,2,15]]}}}