{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,5]],"date-time":"2025-10-05T16:59:15Z","timestamp":1759683555365},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"1","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1996,2,15]]},"abstract":"<jats:p>The effects of ethanol and other aliphatic alcohols on the endoplasmic reticulum Ca2+ pump and the inositol 1,4,5-trisphosphate (InsP3)-sensitive Ca2+ channel were studied in pig cerebellar microsomes. Methanol, ethanol and propanol all stimulated ATP-dependent Ca2+ uptake, whereas butanol inhibited this process. Ethanol inhibited InsP3-induced Ca2+ release [half-maximal inhibition at 3.5%, v\/v (600 mM)]. However, ethanol affected only the amount of InsP3-releasable Ca2+, without affecting the concentration of InsP3 required to induce half-maximal release. Other alcohols of longer chain length were more potent than ethanol at inhibiting InsP3-induced Ca2+ release, but none of the alcohols tested affected [3H]InsP3 binding to its receptor. Using stopped-flow techniques, measurements of the rate of InsP3-induced Ca2+ release in the preparation of pig cerebellar microsomes used in this study showed the kinetics to be monophasic, with a rate constant of 0.93 s-1 at 20 \u03bcM InsP3. This rate constant was dependent upon InsP3 concentration, decreasing to 0.38 s-1 at 0.25 \u03bcM InsP3. Ethanol was shown to reduce the fractional amount of InsP3-induced Ca2+ release without significantly affecting the rate constant for this process.<\/jats:p>","DOI":"10.1042\/bj3140175","type":"journal-article","created":{"date-parts":[[2015,8,10]],"date-time":"2015-08-10T21:47:57Z","timestamp":1439243277000},"page":"175-179","update-policy":"http:\/\/dx.doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Inhibition of the cerebellar inositol 1,4,5-trisphosphate-sensitive Ca2+ channel by ethanol and other aliphatic alcohols"],"prefix":"10.1042","volume":"314","author":[{"given":"Mokdad","family":"MEZNA","sequence":"first","affiliation":[{"name":"School of Biochemistry, University of Birmingham, Edgbaston, Birmingham B15 2TT, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tracy","family":"PATCHICK","sequence":"additional","affiliation":[{"name":"School of Biochemistry, University of Birmingham, Edgbaston, Birmingham B15 2TT, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stephen","family":"TOVEY","sequence":"additional","affiliation":[{"name":"School of Biochemistry, University of Birmingham, Edgbaston, Birmingham B15 2TT, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Francesco","family":"MICHELANGELI","sequence":"additional","affiliation":[{"name":"School of Biochemistry, University of Birmingham, Edgbaston, Birmingham B15 2TT, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","container-title":["Biochemical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/314\/1\/175\/618694\/bj3140175.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/314\/1\/175\/618694\/bj3140175.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,23]],"date-time":"2021-11-23T23:27:13Z","timestamp":1637710033000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/biochemj\/article\/314\/1\/175\/31706\/Inhibition-of-the-cerebellar-inositol-1-4-5"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1996,2,15]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1996,2,15]]}},"URL":"https:\/\/doi.org\/10.1042\/bj3140175","relation":{},"ISSN":["0264-6021","1470-8728"],"issn-type":[{"value":"0264-6021","type":"print"},{"value":"1470-8728","type":"electronic"}],"subject":[],"published":{"date-parts":[[1996,2,15]]}}}