{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T03:38:34Z","timestamp":1761709114948},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"2","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1996,4,15]]},"abstract":"<jats:p>Mitochondria were isolated from biopsies of the biceps femoris muscle of Danish landrace pigs. Three groups of animals were compared: (1) normal pigs; (2) pigs that were homozygous with respect to the gene Haln\/Haln coding for the porcine malignant hyperthermia syndrome; and (3) heterozygote animals. A newly developed micro-method for preparation and assaying of small quantities of intact mitochondria was employed. With this technique mitochondria from biopsies weighing less than 100 mg were examined with respect to cytochrome content as well as phosphorylating and respiratory activities, including the non-phosphorylating exo-NADH oxidase activity. The mitochondria, prepared in a yield of 48%, showed high respiratory activities with tricarboxylic acid-cycle intermediates and pyruvate, and somewhat lower activity with palmitoyl-carnitine as substrate. The ATP synthase activity was about 1000 \u03bcmol ATP\/min per g of protein and the maximal respiratory activity approx. 700 \u03bcmol of O2\/min per g of protein. No differences among the three groups of animals were detected, except for the exo-NADH oxidase activities, which were 43, 78 and 107 \u03bcmol of O2\/ min per g of protein in the groups of normal, heterozygous and homozygous animals respectively. It is concluded that the exo-NADH oxidase activity may be a genetic manifestation of malignant hyperthermia and may play a significant role in the heat production characteristic of the syndrome.<\/jats:p>","DOI":"10.1042\/bj3150659","type":"journal-article","created":{"date-parts":[[2015,8,10]],"date-time":"2015-08-10T21:48:38Z","timestamp":1439243318000},"page":"659-663","update-policy":"http:\/\/dx.doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Characterization of mitochondria from pig muscle: higher activity of exo-NADH oxidase in animals suffering from malignant hyperthermia"],"prefix":"10.1042","volume":"315","author":[{"given":"Ulla F.","family":"RASMUSSEN","sequence":"first","affiliation":[{"name":"The Copenhagen Muscle Research Centre, Department of Biochemistry, August Krogh Institute, University of Copenhagen, Copenhagen"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hans N.","family":"RASMUSSEN","sequence":"additional","affiliation":[{"name":"The Copenhagen Muscle Research Centre, Department of Biochemistry, August Krogh Institute, University of Copenhagen, Copenhagen"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anette J.","family":"ANDERSEN","sequence":"additional","affiliation":[{"name":"The Copenhagen Muscle Research Centre, Department of Biochemistry, August Krogh Institute, University of Copenhagen, Copenhagen"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Preben FOGD","family":"J\u00d8RGENSEN","sequence":"additional","affiliation":[{"name":"The Veterinary University, Copenhagen"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bj\u2298rn","family":"QUISTORFF","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Genetics, NMR Center, Panum Institute, University of Copenhagen, Copenhagen, Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","container-title":["Biochemical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/315\/2\/659\/619848\/bj3150659.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/315\/2\/659\/619848\/bj3150659.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,24]],"date-time":"2021-11-24T00:16:12Z","timestamp":1637712972000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/biochemj\/article\/315\/2\/659\/32946\/Characterization-of-mitochondria-from-pig-muscle"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1996,4,15]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1996,4,15]]}},"URL":"https:\/\/doi.org\/10.1042\/bj3150659","relation":{},"ISSN":["0264-6021","1470-8728"],"issn-type":[{"value":"0264-6021","type":"print"},{"value":"1470-8728","type":"electronic"}],"subject":[],"published":{"date-parts":[[1996,4,15]]}}}