{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T04:01:37Z","timestamp":1791086497087,"version":"4.1.0"},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"1","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1996,7,1]]},"abstract":"<jats:p>Specific binding of 125I-labelled bovine odour-binding protein (OBP) to isolated membranes from nasal mucosa was demonstrated. The interaction reached equilibrium within 30 min at 37 \u00b0C and was reversible. A Scatchard analysis of the equilibrium binding revealed a single population of binding sites, with the calculated equilibrium dissociation constant and maximum number of binding sites being 2.25\u00b10.5 \u03bcM and 18.5\u00b12 pmol\/mg of membrane protein respectively (n = 2). Receptor activity was decreased on digestion by trypsin, proteinase K or endoglycosidase H, was heat labile and was sensitive to thiol-group-specific reagents. With the exception of rat and mouse major urinary proteins, which exhibit a high degree of structural similarity with OBP and bind similar ligands, other members of the lipocalin family, such as retinol-binding protein and \u03b2-lactoglobulin, failed to inhibit the binding of 125I-labelled OBP to its receptor. The receptor seems not to be restricted to olfactory tissues, as it was detected in a variety of other tissues. This suggests that OBP is unlikely to play a role only in olfactory signal transduction. It might have a much broader role within the body; possibilities include a role in detoxification or signalling.<\/jats:p>","DOI":"10.1042\/bj3170023","type":"journal-article","created":{"date-parts":[[2015,8,10]],"date-time":"2015-08-10T21:49:58Z","timestamp":1439243398000},"page":"23-27","update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":32,"title":["Membrane receptor for odour-binding proteins"],"prefix":"10.1042","volume":"317","author":[{"given":"Mohamed","family":"BOUDJELAL","sequence":"first","affiliation":[{"name":"Department of Biochemistry and Molecular Biology"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Asipu","family":"SIVAPRASADARAO","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, University of Leeds, Leeds LS2 9JT, U.K."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John B. C","family":"FINDLAY","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"288","container-title":["Biochemical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/317\/1\/23\/621174\/bj3170023.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/317\/1\/23\/621174\/bj3170023.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,23]],"date-time":"2021-11-23T23:30:14Z","timestamp":1637710214000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/biochemj\/article\/317\/1\/23\/31806\/Membrane-receptor-for-odour-binding-proteins"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1996,7,1]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1996,7,1]]}},"URL":"https:\/\/doi.org\/10.1042\/bj3170023","relation":{},"ISSN":["0264-6021","1470-8728"],"issn-type":[{"value":"0264-6021","type":"print"},{"value":"1470-8728","type":"electronic"}],"subject":[],"published":{"date-parts":[[1996,7,1]]}}}