{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T09:14:26Z","timestamp":1790241266811,"version":"4.1.0"},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"3","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1996,9,15]]},"abstract":"<jats:p>Hormone-sensitive lipase (HSL) catalyses the rate-limiting step in adipocyte lipolysis. Short-term hormonal regulation of HSL activity is well characterized, whereas little is known about the control of HSL gene expression. We have measured HSL mRNA content of 3T3-F442A and BFC-1 adipocytes in response to the cAMP analogue 8-(4-chlorophenylthio)-cAMP (8-CPT-cAMP) and to the phorbol ester phorbol 12-myristate 13-acetate (PMA) by Northern blot, using a specific mouse cDNA fragment. Treatment of the cells for 12 or 6 h with, respectively, 0.5 mM 8-CPT-cAMP or 1 \u00b5M PMA produced a maximal decrease of about 60% in HSL mRNA. These effects were unaffected by the protein-synthesis inhibitor anisomycin, suggesting that cAMP and PMA actions were direct. The reduction in HSL mRNA was accompanied by a reduction in HSL total activity. The intracellular routes that cAMP and PMA follow for inducing such an effect seemed clearly independent. (i) After desensitization of the protein kinase C regulation pathway by a 24 h treatment of the cells with 1 \u00b5M PMA, PMA action was abolished whereas cAMP was still fully active. (ii) Treatment with saturating concentrations of both agents produced an additive effect. (iii) The synthetic glucocorticoid dexamethasone had no proper effect on HSL gene expression but potentiated cAMP action without affecting PMA action. cAMP inhibitory action on HSL is unexpected. Indeed, the second messenger of catecholamines is the main activator of HSL by phosphorylation. We envision that a long-term cAMP treatment of adipocytes induces a counter-regulatory process that reduces HSL content and, ultimately, limits fatty acid depletion from stored triacylglycerols.<\/jats:p>","DOI":"10.1042\/bj3181057","type":"journal-article","created":{"date-parts":[[2015,8,10]],"date-time":"2015-08-10T21:50:33Z","timestamp":1439243433000},"page":"1057-1063","update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":27,"title":["Inhibition of hormone-sensitive lipase gene expression by cAMP and phorbol esters in 3T3-F442A and BFC-1 adipocytes"],"prefix":"10.1042","volume":"318","author":[{"given":"Emmanuelle","family":"PL\u00c9E-GAUTIER","sequence":"first","affiliation":[{"name":"Centre de Recherche sur l'Endocrinologie Mol\u00e9culaire et le D\u00e9veloppement, C.N.R.S., 9 rue Jules Hetzel, 92190 Meudon, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jacques","family":"GROBER","sequence":"additional","affiliation":[{"name":"INSERM Unit\u00e9 317, Institut Louis Bugnard, B\u00e2timent L3, Facult\u00e9 de M\u00e9decine, H\u00f4pital Rangueil, F-31054 Toulouse Cedex, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric","family":"DUPLUS","sequence":"additional","affiliation":[{"name":"Centre de Recherche sur l'Endocrinologie Mol\u00e9culaire et le D\u00e9veloppement, C.N.R.S., 9 rue Jules Hetzel, 92190 Meudon, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dominique","family":"LANGIN","sequence":"additional","affiliation":[{"name":"INSERM Unit\u00e9 317, Institut Louis Bugnard, B\u00e2timent L3, Facult\u00e9 de M\u00e9decine, H\u00f4pital Rangueil, F-31054 Toulouse Cedex, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claude","family":"FOREST","sequence":"additional","affiliation":[{"name":"Centre de Recherche sur l'Endocrinologie Mol\u00e9culaire et le D\u00e9veloppement, C.N.R.S., 9 rue Jules Hetzel, 92190 Meudon, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"288","container-title":["Biochemical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/318\/3\/1057\/621630\/bj3181057.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/318\/3\/1057\/621630\/bj3181057.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,24]],"date-time":"2021-11-24T00:19:00Z","timestamp":1637713140000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/biochemj\/article\/318\/3\/1057\/33023\/Inhibition-of-hormone-sensitive-lipase-gene"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1996,9,15]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1996,9,15]]}},"URL":"https:\/\/doi.org\/10.1042\/bj3181057","relation":{},"ISSN":["0264-6021","1470-8728"],"issn-type":[{"value":"0264-6021","type":"print"},{"value":"1470-8728","type":"electronic"}],"subject":[],"published":{"date-parts":[[1996,9,15]]}}}