{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,9]],"date-time":"2025-11-09T03:37:01Z","timestamp":1762659421044},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"1","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1996,11,15]]},"abstract":"<jats:p>Disulfiram [bis(diethylthiocarbamoyl)disulphide] has been found to stimulate reversibly the Ca2+-ATPase of skeletal muscle sarcoplasmic reticulum. At pH 7.2, 2.1 mM ATP and 25 \u00b0C, ATPase activity was found to double on addition of 120 \u00b5M disulfiram. Stimulation fitted to binding of disulfiram at a single site with a Kd of 61 \u00b5M. Disulfiram had no effect on the Ca2+ affinity of the ATPase or on the rate of phosphorylation of the ATPase by ATP, but increased the rate of dissociation of Ca2+ from the phosphorylated ATPase (the transport step) and increased the rate of dephosphorylation of the phosphorylated ATPase. It also decreased the level of phosphorylation of the ATPase by Pi, consistent with a 7.5-fold decrease in the equilibrium constant of the phosphorylated to non-phosphorylated forms (E2PMg\/E2PiMg) at 80 \u00b5M disulfiram. Disulfiram had no significant effect on the concentration of ATP resulting in stimulation of ATPase activity, suggesting that it does not bind to the empty nucleotide-binding site on the phosphorylated ATPase. Studies of the effects of mixtures of disulfiram and jasmone (another molecule that stimulates the ATPase) suggest that they bind to separate sites on the ATPase.<\/jats:p>","DOI":"10.1042\/bj3200101","type":"journal-article","created":{"date-parts":[[2015,8,10]],"date-time":"2015-08-10T21:51:59Z","timestamp":1439243519000},"page":"101-105","update-policy":"http:\/\/dx.doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Stimulation of the Ca2+-ATPase of sarcoplasmic reticulum by disulfiram"],"prefix":"10.1042","volume":"320","author":[{"given":"Anthony P","family":"STARLING","sequence":"first","affiliation":[{"name":"Department of Biochemistry and Institute for Biomolecular Sciences, University of Southampton, Biomedical Sciences Building, Southampton SO16 7PX, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"Malcolm EAST","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Institute for Biomolecular Sciences, University of Southampton, Biomedical Sciences Building, Southampton SO16 7PX, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anthony G","family":"LEE","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Institute for Biomolecular Sciences, University of Southampton, Biomedical Sciences Building, Southampton SO16 7PX, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","container-title":["Biochemical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/320\/1\/101\/622643\/bj3200101.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/320\/1\/101\/622643\/bj3200101.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,24]],"date-time":"2021-11-24T00:22:36Z","timestamp":1637713356000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/biochemj\/article\/320\/1\/101\/33228\/Stimulation-of-the-Ca2-ATPase-of-sarcoplasmic"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1996,11,15]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1996,11,15]]}},"URL":"https:\/\/doi.org\/10.1042\/bj3200101","relation":{},"ISSN":["0264-6021","1470-8728"],"issn-type":[{"value":"0264-6021","type":"print"},{"value":"1470-8728","type":"electronic"}],"subject":[],"published":{"date-parts":[[1996,11,15]]}}}