{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,3,6]],"date-time":"2023-03-06T09:53:32Z","timestamp":1678096412349},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"2","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1996,12,1]]},"abstract":"<jats:p>Complete cDNA and genomic sequences encoding the Onchocerca volvulus S-adenosylmethionine decarboxylase (SAMDC), a key enzyme in polyamine biosynthesis, have been isolated and characterized. The deduced amino acid sequence encodes a 42 kDa proenzyme with a moderate level of sequence homology to eukaryotic SAMDCs. Enzymically active O. volvulusSAMDC was expressed at a high level in an Escherichia colimutant strain lacking endogenous SAMDC. The recombinant enzyme was purified to homogeneity using DEAE-cellulose, methylglyoxal bis(guanylhydrazone)\u2013Sepharose and Superdex S-200 chromatography. It was determined that the recombinant proenzyme is cleaved to produce 32 and 10 kDa subunits. The sequence of the N-terminal portion of the large subunit was determined and comparison with the sequence of the proenzyme revealed that the precise cleavage site lies between Glu86 and Ser87. Gel-filtration experiments demonstrated that these two subunits combine to form an active heterotetramer. Comparison of the cDNA and genomic sequences revealed that the SAMDC mRNA undergoes both cis- and trans-splicing in its 5\u00b4-untranslated region (UTR). Anchored PCR on O. volvulusmRNA confirmed the cDNA sequence and identified two distinct trans-spliced products, a 22-nucleotide spliced-leader sequence and a 138 bp sequence containing the 22 nucleotide spliced-leader sequence. Genomic Southern-blot analysis suggests that the O. volvulusSAMDC is encoded by a single-copy gene. This gene spans 5.3 kb and is comprised of nine exons and eight introns. The first intron is located in the 5\u00b4-UTR and processing of this intron has a potential regulatory function. The 5\u00b4-flanking region of the gene contains potential transcriptional regulatory elements such as a TATA box, two CAAT boxes and AP-1-, C\/EBP-, ELP-, H-APF-1-, HNF-5- and PEA3-binding sites.<\/jats:p>","DOI":"10.1042\/bj3200519","type":"journal-article","created":{"date-parts":[[2015,8,10]],"date-time":"2015-08-10T21:51:59Z","timestamp":1439243519000},"page":"519-530","update-policy":"http:\/\/dx.doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["A novel <i>trans<\/i>-spliced mRNA from <i>Onchocerca volvulus<\/i> encodes a functional S-adenosylmethionine decarboxylase"],"prefix":"10.1042","volume":"320","author":[{"given":"Akram A.","family":"DA'DARA","sequence":"first","affiliation":[{"name":"Bernhard Nocht Institute for Tropical Medicine, Department of Biochemistry, Bernhard Nocht Strasse 74, D-20359 Hamburg, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kimberly","family":"HENKLE-D\u00dcHRSEN","sequence":"additional","affiliation":[{"name":"Bernhard Nocht Institute for Tropical Medicine, Department of Biochemistry, Bernhard Nocht Strasse 74, D-20359 Hamburg, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rolf D.","family":"WALTER","sequence":"additional","affiliation":[{"name":"Bernhard Nocht Institute for Tropical Medicine, Department of Biochemistry, Bernhard Nocht Strasse 74, D-20359 Hamburg, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","container-title":["Biochemical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/320\/2\/519\/623131\/bj3200519.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/320\/2\/519\/623131\/bj3200519.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,24]],"date-time":"2021-11-24T01:06:28Z","timestamp":1637715988000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/biochemj\/article\/320\/2\/519\/37907\/A-novel-trans-spliced-mRNA-from-Onchocerca"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1996,12,1]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1996,12,1]]}},"URL":"https:\/\/doi.org\/10.1042\/bj3200519","relation":{},"ISSN":["0264-6021","1470-8728"],"issn-type":[{"value":"0264-6021","type":"print"},{"value":"1470-8728","type":"electronic"}],"subject":[],"published":{"date-parts":[[1996,12,1]]}}}