{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T17:30:53Z","timestamp":1791307853262,"version":"4.3.1"},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"2","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1997,4,15]]},"abstract":"<jats:p>Echicetin, a heterodimeric protein from the venom of Echis carinatus, binds to platelet glycoprotein Ib (GPIb) and so inhibits platelet aggregation or agglutination induced by various platelet agonists acting via GPIb. The amino acid sequence of the \u03b2 subunit of echicetin has been reported and found to belong to the recently identified snake venom subclass of the C-type lectin protein family. Echicetin \u03b1 and \u03b2 subunits were purified. N-terminal sequence analysis provided direct evidence that the protein purified was echicetin. The paper presents the complete amino acid sequence of the \u03b1 subunit and computer models of the \u03b1 and \u03b2 subunits. The sequence of \u03b1 echicetin is highly similar to the \u03b1 and \u03b2 chains of various heterodimeric and homodimeric C-type lectins. Neither of the fully reduced and alkylated \u03b1 or \u03b2 subunits of echicetin inhibited the platelet agglutination induced by von Willebrand factor\u2013ristocetin or \u03b1-thrombin. Earlier reports about the inhibitory activity of reduced and alkylated echicetin \u03b2 subunit might have been due to partial reduction of the protein.<\/jats:p>","DOI":"10.1042\/bj3230533","type":"journal-article","created":{"date-parts":[[2015,8,10]],"date-time":"2015-08-10T21:54:07Z","timestamp":1439243647000},"page":"533-537","update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":37,"title":["Amino acid sequence of the \u03b1 subunit and computer modelling of the \u03b1 and <i>\u03b2<\/i> subunits of echicetin from the venom of <i>Echis carinatus<\/i> (saw-scaled viper)"],"prefix":"10.1042","volume":"323","author":[{"given":"J\u00e1nos","family":"POLG\u00c1R","sequence":"first","affiliation":[{"name":"Theodor Kocher Institute, University of Berne, Freiestrasse 1, CH-3012 Berne, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edith M.","family":"MAGNENAT","sequence":"additional","affiliation":[{"name":"Geneva Biomedical Research Institute, Glaxo Wellcome Research and Development S. A., 14, chemin des Aulx, CH-1228 Plan-les-Ouates, Geneva, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manuel C.","family":"PEITSCH","sequence":"additional","affiliation":[{"name":"Geneva Biomedical Research Institute, Glaxo Wellcome Research and Development S. A., 14, chemin des Aulx, CH-1228 Plan-les-Ouates, Geneva, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Timothy N. C.","family":"WELLS","sequence":"additional","affiliation":[{"name":"Geneva Biomedical Research Institute, Glaxo Wellcome Research and Development S. A., 14, chemin des Aulx, CH-1228 Plan-les-Ouates, Geneva, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mansoor S. A.","family":"SAQI","sequence":"additional","affiliation":[{"name":"Glaxo Wellcome Research and Development, Stevenage SG1 2NY, U.K."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kenneth J.","family":"CLEMETSON","sequence":"additional","affiliation":[{"name":"Theodor Kocher Institute, University of Berne, Freiestrasse 1, CH-3012 Berne, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"288","container-title":["Biochemical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/323\/2\/533\/624220\/bj3230533.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/323\/2\/533\/624220\/bj3230533.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,24]],"date-time":"2021-11-24T00:04:45Z","timestamp":1637712285000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/biochemj\/article\/323\/2\/533\/32715\/Amino-acid-sequence-of-the-subunit-and-computer"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1997,4,15]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1997,4,15]]}},"URL":"https:\/\/doi.org\/10.1042\/bj3230533","relation":{},"ISSN":["0264-6021","1470-8728"],"issn-type":[{"value":"0264-6021","type":"print"},{"value":"1470-8728","type":"electronic"}],"subject":[],"published":{"date-parts":[[1997,4,15]]}}}