{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T15:19:40Z","timestamp":1761059980857},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"3","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1997,6,15]]},"abstract":"<jats:p>The A-Myb transcription factor belongs to the Myb family of oncoproteins and is likely to be involved in the regulation of proliferation and\/or differentiation of normal B cells and Burkitt's lymphoma cells. To characterize in detail the domains of A-Myb that regulate its function, we have generated a series of deletion mutants and have investigated their trans-activation potential as well as their DNA-binding activity. Our results have allowed us to delineate the trans-activation domain as well as two separate regulatory regions. The boundaries of the trans-activation domain (amino acid residues 218\u2013319) are centred on a sequence rich in charged amino acids (residues 259\u2013281). A region (residues 320\u2013482) localized immediately downstream of the trans-activation domain and containing a newly identified conserved stretch of 48 residues markedly inhibits specific DNA binding. Finally the last 110 residues of A-Myb (residues 643\u2013752), which include a sequence conserved in all mammalian myb genes (region III), negatively regulate the maximal trans-activation potential of A-Myb. We have also investigated the functional interaction between A-Myb and the nuclear adaptor molecule CBP [cAMP response element-binding protein (CREB)-binding protein]. We demonstrate that CBP synergizes with A-Myb in a dose-dependent fashion, and that this co-operative effect can be inhibited by E1A and can also be observed with the CBP homologue p300. We show that this functional synergism requires the presence of the A-Myb charged sequence and that it involves physical interaction between A-Myb and the CREB-binding domain of CBP.<\/jats:p>","DOI":"10.1042\/bj3240729","type":"journal-article","created":{"date-parts":[[2015,8,10]],"date-time":"2015-08-10T21:54:41Z","timestamp":1439243681000},"page":"729-736","update-policy":"http:\/\/dx.doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":38,"title":["Regulatory domains of the A-Myb transcription factor and its interaction with the CBP\/p300 adaptor molecules"],"prefix":"10.1042","volume":"324","author":[{"given":"Valeria","family":"FACCHINETTI","sequence":"first","affiliation":[{"name":"Laboratory of Molecular Immunohematology, Istituto Ricerche Farmacologiche \u2018Mario Negri\u2019, via Eritrea 62, 20157 Milano, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Livio","family":"LOFFARELLI","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Immunohematology, Istituto Ricerche Farmacologiche \u2018Mario Negri\u2019, via Eritrea 62, 20157 Milano, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sabine","family":"SCHREEK","sequence":"additional","affiliation":[{"name":"Institut f\u00fcr Molekularbiologie OE5250, Medizinische Hochschule Hannover, 30623 Hannover, Federal Republic of Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael","family":"OELGESCHL\u00c4GER","sequence":"additional","affiliation":[{"name":"Institut f\u00fcr Molekularbiologie OE5250, Medizinische Hochschule Hannover, 30623 Hannover, Federal Republic of Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bernhard","family":"L\u00dcSCHER","sequence":"additional","affiliation":[{"name":"Institut f\u00fcr Molekularbiologie OE5250, Medizinische Hochschule Hannover, 30623 Hannover, Federal Republic of Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Martino","family":"INTRONA","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Immunohematology, Istituto Ricerche Farmacologiche \u2018Mario Negri\u2019, via Eritrea 62, 20157 Milano, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jos\u00e9e","family":"GOLAY","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Immunohematology, Istituto Ricerche Farmacologiche \u2018Mario Negri\u2019, via Eritrea 62, 20157 Milano, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","container-title":["Biochemical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/324\/3\/729\/624784\/bj3240729.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/324\/3\/729\/624784\/bj3240729.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,24]],"date-time":"2021-11-24T00:29:33Z","timestamp":1637713773000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/biochemj\/article\/324\/3\/729\/33775\/Regulatory-domains-of-the-A-Myb-transcription"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1997,6,15]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1997,6,15]]}},"URL":"https:\/\/doi.org\/10.1042\/bj3240729","relation":{},"ISSN":["0264-6021","1470-8728"],"issn-type":[{"value":"0264-6021","type":"print"},{"value":"1470-8728","type":"electronic"}],"subject":[],"published":{"date-parts":[[1997,6,15]]}}}