{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,6,27]],"date-time":"2025-06-27T10:37:57Z","timestamp":1751020677217},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"1","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1997,7,1]]},"abstract":"<jats:p>Protein kinase C (PKC) has been implicated in interleukin 1 (IL1) signal transduction in a number of cellular systems, either as a key event in IL1 action or as a negative regulator. Here we have examined the effects of two PKC inhibitors, staurosporine and the more selective agent Ro 31-8220, on IL1 responses in the murine thymoma line EL4.NOB-1. A 1 h pulse of staurosporine was found to strongly potentiate the induction of IL2 by IL1\u03b1 in these cells. In contrast, neither a pulse nor prolonged incubation with Ro 31-8220 affected the response to IL1\u03b1. Both agents blocked the response to PMA, however. A 1 h pulse of staurosporine was also found to induce IL2 production on its own, activate the transcription factor nuclear factor \u03baB (NF\u03baB) and increase the expression of a NF\u03baB-linked reporter gene. It synergized with IL1\u03b1 in all of these responses. Ro 31-8220 was again without effect, although both staurosporine and Ro 31-8220 blocked the activation of NF\u03baB by PMA. Finally, staurosporine caused the translocation of PKC-\u03b1 and -\u03f5, and to a lesser extent PKC-\u03b2, but not PKC-\u03b8 or -\u03b6, from the cytosol to the membrane, although a similar effect was observed with Ro 31-8220. The results suggest that PKC is not involved in IL1\u03b1 signalling in EL4 cells. Furthermore, the potentiating effect of staurosporine on IL1\u03b1 action does not involve PKC inhibition, and is likely to be at the level of NF\u03baB activation.<\/jats:p>","DOI":"10.1042\/bj3250039","type":"journal-article","created":{"date-parts":[[2015,8,10]],"date-time":"2015-08-10T21:55:16Z","timestamp":1439243716000},"page":"39-45","update-policy":"http:\/\/dx.doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Staurosporine, but not Ro 31-8220, induces interleukin 2 production and synergizes with interleukin 1<i>\u03b1<\/i> in EL4 thymoma cells: Activation of nuclear factor <i>\u03ba<\/i>B as a common signal for staurosporine and interleukin 1<i>\u03b1<\/i>"],"prefix":"10.1042","volume":"325","author":[{"given":"Tara M.","family":"MOHAN","sequence":"first","affiliation":[{"name":"Department of Biochemistry, Trinity College Dublin, Dublin 2, Ireland"},{"name":"Department of Biochemistry, Trinity College Dublin, Dublin 2, Ireland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jane S.","family":"MATTHEWS","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Trinity College Dublin, Dublin 2, Ireland"},{"name":"Department of Biochemistry, Trinity College Dublin, Dublin 2, Ireland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luke A. J.","family":"O'NEILL","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Trinity College Dublin, Dublin 2, Ireland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","container-title":["Biochemical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/325\/1\/39\/624406\/bj3250039.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/325\/1\/39\/624406\/bj3250039.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,24]],"date-time":"2021-11-24T00:29:59Z","timestamp":1637713799000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/biochemj\/article\/325\/1\/39\/33812\/Staurosporine-but-not-Ro-31-8220-induces"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1997,7,1]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1997,7,1]]}},"URL":"https:\/\/doi.org\/10.1042\/bj3250039","relation":{},"ISSN":["0264-6021","1470-8728"],"issn-type":[{"value":"0264-6021","type":"print"},{"value":"1470-8728","type":"electronic"}],"subject":[],"published":{"date-parts":[[1997,7,1]]}}}