{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T02:04:53Z","timestamp":1791079493255,"version":"4.1.0"},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"1","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1998,2,15]]},"abstract":"<jats:p>We have demonstrated previously that isolated guinea-pig alveolar macrophages (AM) synthesize type-II phospholipase A2 (PLA2-II) through a tumour necrosis factor-\u03b1 (TNF-\u03b1)-dependent process. This synthesis is enhanced by lipopolysaccharide (LPS) and accompanied by a release of prostaglandin E2 (PGE2) into the medium. Because agents elevating intracellular cAMP, such as PGE2, have been shown to stimulate PLA2-II expression in various cell types, we investigated the modulation of PLA2-II synthesis by cAMP in AM. Surprisingly, incubation of AM with PGE2, dibutyryl-cAMP, cholera toxin or rolipram (an inhibitor of specific cAMP-phosphodiesterase) inhibited both basal and LPS-stimulated PLA2-II expression. The inhibitory effect of PGE2 was observed at concentrations similar to those released by AM. Moreover, treatment of AM with either aspirin or neutralizing PGE2 monoclonal antibody stimulated PLA2-II synthesis. These effects were closely correlated with the ability of these agents to modulate TNF-\u03b1 release, which was decreased by dibutyryl-cAMP and exogenous PGE2, whereas neutralizing PGE2 antibody markedly increased this release. Hence, in contrast to other cell systems, we report that: (i) agents elevating intracellular cAMP levels down-regulate both basal and LPS-induced PLA2-II synthesis, (ii) prostaglandins exert a negative feedback effect on this synthesis, probably through an elevation of intracellular cAMP levels, and (iii) inhibition of TNF-\u03b1 release may account, at least in part, for the down-regulation of PLA2-II expression by endogenously produced prostaglandins and cAMP-elevating agents.<\/jats:p>","DOI":"10.1042\/bj3300089","type":"journal-article","created":{"date-parts":[[2015,8,10]],"date-time":"2015-08-10T22:00:40Z","timestamp":1439244040000},"page":"89-94","update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Down-regulation by prostaglandins of type-II phospholipase A2 expression in guinea-pig alveolar macrophages: a possible involvement of cAMP"],"prefix":"10.1042","volume":"330","author":[{"given":"Daniel","family":"VIAL","sequence":"first","affiliation":[{"name":"Unit\u00e9 de Pharmacologie Cellulaire, Unit\u00e9 Associ\u00e9e Pasteur\/INSERM U285, Institut Pasteur, 25 rue Dr. Roux 75015 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laurence","family":"ARBIBE","sequence":"additional","affiliation":[{"name":"Unit\u00e9 de Pharmacologie Cellulaire, Unit\u00e9 Associ\u00e9e Pasteur\/INSERM U285, Institut Pasteur, 25 rue Dr. Roux 75015 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nathalie","family":"HAVET","sequence":"additional","affiliation":[{"name":"Unit\u00e9 de Pharmacologie Cellulaire, Unit\u00e9 Associ\u00e9e Pasteur\/INSERM U285, Institut Pasteur, 25 rue Dr. Roux 75015 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claude","family":"DUMAREY","sequence":"additional","affiliation":[{"name":"Unit\u00e9 de Pharmacologie Cellulaire, Unit\u00e9 Associ\u00e9e Pasteur\/INSERM U285, Institut Pasteur, 25 rue Dr. Roux 75015 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B. Boris","family":"VARGAFTIG","sequence":"additional","affiliation":[{"name":"Unit\u00e9 de Pharmacologie Cellulaire, Unit\u00e9 Associ\u00e9e Pasteur\/INSERM U285, Institut Pasteur, 25 rue Dr. Roux 75015 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lhousseine","family":"TOUQUI","sequence":"additional","affiliation":[{"name":"Unit\u00e9 de Pharmacologie Cellulaire, Unit\u00e9 Associ\u00e9e Pasteur\/INSERM U285, Institut Pasteur, 25 rue Dr. Roux 75015 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"288","container-title":["Biochemical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/330\/1\/89\/634277\/bj3300089.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/330\/1\/89\/634277\/bj3300089.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,24]],"date-time":"2021-11-24T00:57:23Z","timestamp":1637715443000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/biochemj\/article\/330\/1\/89\/36939\/Down-regulation-by-prostaglandins-of-type-II"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1998,2,15]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1998,2,15]]}},"URL":"https:\/\/doi.org\/10.1042\/bj3300089","relation":{},"ISSN":["0264-6021","1470-8728"],"issn-type":[{"value":"0264-6021","type":"print"},{"value":"1470-8728","type":"electronic"}],"subject":[],"published":{"date-parts":[[1998,2,15]]}}}