{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T20:31:27Z","timestamp":1791405087217,"version":"4.3.3"},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"1","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1998,2,15]]},"abstract":"<jats:p>We examined the effect of a single injection of 3,5-di-iodo-l-thyronine (3,5-T2) (150 \u03bcg\/100 g body weight) on the rat liver mitochondrial energy-transduction apparatus. We applied \u2018top-down\u2019 elasticity analysis, which allows identification of the site of action of an effector within a metabolic pathway. This kinetic approach considers oxidative phosphorylation as two blocks of reactions: those generating the mitochondrial inner-membrane potential (\u0394\u03c8; \u2018substrate oxidation\u2019) and those \u2018consuming\u2019 it (\u2018proton leak\u2019 and \u2018phosphorylating system\u2019). The results show that 1 h after the injection of 3,5-T2, state 4 (respiratory state in which there is no ATP synthesis and the exogenous ADP added has been exhausted) and state 3 (respiratory state in which ATP synthesis is at maximal rate) of mitochondrial respiration were significantly increased (by approx. 30%). \u2018Top-down\u2019 elasticity analysis revealed that these increases were due to the stimulation of reactions involved in substrate oxidation; neither \u2018proton leak\u2019 nor the \u2018phosphorylating system\u2019 was influenced by 3,5-T2. Using the same approach we divided the respiratory chain into two blocks of reactions: cytochrome c reducers and cytochrome c oxidizers. We found that both cytochrome c reducers and cytochrome c oxidizers are targets for 3,5-T2. The rapidity with which 3,5-T2 acts in stimulating the mitochondrial respiration rate suggests to us that di-iodo-L-thyronine may play an important role in the physiological conditions in which rapid energy utilization is required, such as cold exposure or overfeeding.<\/jats:p>","DOI":"10.1042\/bj3300521","type":"journal-article","created":{"date-parts":[[2015,8,10]],"date-time":"2015-08-10T22:00:40Z","timestamp":1439244040000},"page":"521-526","update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":51,"title":["Effect of 3,5-di-iodo-L-thyronine on the mitochondrial energy-transduction apparatus"],"prefix":"10.1042","volume":"330","author":[{"given":"Assonta","family":"LOMBARDI","sequence":"first","affiliation":[{"name":"Dipartimento di Fisiologia Generale ed Ambientale, Universit\u00e0 di Napoli \u2018Federico II\u2019, Via Mezzocannone, 8, 80134 Napoli, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonia","family":"LANNI","sequence":"additional","affiliation":[{"name":"Dipartimento di Fisiologia Generale ed Ambientale, Universit\u00e0 di Napoli \u2018Federico II\u2019, Via Mezzocannone, 8, 80134 Napoli, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria","family":"MORENO","sequence":"additional","affiliation":[{"name":"Dipartimento di Chimica, Facolt\u00e0 di Scienze, Universit\u00e0 degli Studi di Salerno, Via S. Allende, 84081 Baronissi, Salerno, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D. Martin","family":"BRAND","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, University of Cambridge, Tennis Court Road, Cambridge CB2 IQW, U.K."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fernando","family":"GOGLIA","sequence":"additional","affiliation":[{"name":"Dipartimento di Fisiologia Generale ed Ambientale, Universit\u00e0 di Napoli \u2018Federico II\u2019, Via Mezzocannone, 8, 80134 Napoli, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"288","container-title":["Biochemical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/330\/1\/521\/634288\/bj3300521.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/330\/1\/521\/634288\/bj3300521.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,24]],"date-time":"2021-11-24T00:57:39Z","timestamp":1637715459000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/biochemj\/article\/330\/1\/521\/36961\/Effect-of-3-5-di-iodo-L-thyronine-on-the"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1998,2,15]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1998,2,15]]}},"URL":"https:\/\/doi.org\/10.1042\/bj3300521","relation":{},"ISSN":["0264-6021","1470-8728"],"issn-type":[{"value":"0264-6021","type":"print"},{"value":"1470-8728","type":"electronic"}],"subject":[],"published":{"date-parts":[[1998,2,15]]}}}