{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T01:40:25Z","timestamp":1777513225050,"version":"3.51.4"},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"1","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1998,4,1]]},"abstract":"<jats:p>Although the oxytocin receptor modulates intracellular Ca2+ ion levels in myometrium, the identities of signal molecules have not been clearly clarified. Our previous studies on oxytocin receptor signalling demonstrated that 80\u00a0kDa Gh\u03b1 is a signal mediator [Baek, Kwon, Lee, Kim, Muralidhar and Im (1996) Biochem. J. 315, 739\u2013744]. To elucidate the effector in the oxytocin receptor signalling pathway, we evaluated the oxytocin-mediated activation of phospholipase C (PLC) by using solubilized membranes from human myometrium and a three-component preparation containing the oxytocin receptor\u2013Gh\u03b1\u2013PLC-\u03b41 complex. PLC-\u03b41 activity in the three-component preparation, as well as PLC activity in solubilized membranes, was increased by oxytocin in the presence of Ca2+ and activated Gh\u03b1 (GTP-bound Gh\u03b1). Furthermore the stimulated PLC-\u03b41 activity resulting from activation of Gh\u03b1 via the oxytocin receptor was significantly attenuated by the selective oxytocin antagonist desGly-NH2d(CH2)5[Tyr(Me)2,Thr4]ornithine vasotocin or GDP. Consistent with these observations, co-immunoprecipitation and co-immunoadsorption of PLC-\u03b41 in the three-component preparation by anti-Gh7\u03b1 antibody resulted in the PLC-\u03b41 being tightly coupled to activated Gh\u03b1 on stimulation of the oxytocin receptor. These results indicate that PLC-\u03b41 is the effector for Gh\u03b1-mediated oxytocin receptor signalling.<\/jats:p>","DOI":"10.1042\/bj3310283","type":"journal-article","created":{"date-parts":[[2015,8,10]],"date-time":"2015-08-10T22:01:27Z","timestamp":1439244087000},"page":"283-289","update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":56,"title":["Phospholipase C-\u03b41 and oxytocin receptor signalling: evidence of its role as an effector"],"prefix":"10.1042","volume":"331","author":[{"given":"Eu-Suk","family":"PARK","sequence":"first","affiliation":[{"name":"Department of Biochemistry, College of Medicine, Chung-Ang University, 221 Heuksuk-Dong, Dongjak-Gu, Seoul 156-756, South Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jong Hyun","family":"WON","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, College of Medicine, Chung-Ang University, 221 Heuksuk-Dong, Dongjak-Gu, Seoul 156-756, South Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kee Jung","family":"HAN","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, College of Medicine, Chung-Ang University, 221 Heuksuk-Dong, Dongjak-Gu, Seoul 156-756, South Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pann-Ghill","family":"SUH","sequence":"additional","affiliation":[{"name":"Department of Life Science, Pohang University of Science and Technology, Pohang 790-784, South Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sung Ho","family":"RYU","sequence":"additional","affiliation":[{"name":"Department of Life Science, Pohang University of Science and Technology, Pohang 790-784, South Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hee Sung","family":"LEE","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, College of Medicine, Chung-Ang University, 221 Heuksuk-Dong, Dongjak-Gu, Seoul 156-756, South Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hye-Young","family":"YUN","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, College of Medicine, Chung-Ang University, 221 Heuksuk-Dong, Dongjak-Gu, Seoul 156-756, South Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nyoun Soo","family":"KWON","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, College of Medicine, Chung-Ang University, 221 Heuksuk-Dong, Dongjak-Gu, Seoul 156-756, South Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kwang Jin","family":"BAEK","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, College of Medicine, Chung-Ang University, 221 Heuksuk-Dong, Dongjak-Gu, Seoul 156-756, South Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","container-title":["Biochemical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/331\/1\/283\/630838\/bj3310283.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/331\/1\/283\/630838\/bj3310283.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,24]],"date-time":"2021-11-24T00:41:26Z","timestamp":1637714486000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/biochemj\/article\/331\/1\/283\/34479\/Phospholipase-C-1-and-oxytocin-receptor-signalling"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1998,4,1]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1998,4,1]]}},"URL":"https:\/\/doi.org\/10.1042\/bj3310283","relation":{},"ISSN":["0264-6021","1470-8728"],"issn-type":[{"value":"0264-6021","type":"print"},{"value":"1470-8728","type":"electronic"}],"subject":[],"published":{"date-parts":[[1998,4,1]]}}}