{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T03:31:48Z","timestamp":1777606308289,"version":"3.51.4"},"reference-count":0,"publisher":"Portland Press Ltd.","issue":"2","content-domain":{"domain":["portlandpress.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[1998,6,1]]},"abstract":"<jats:p>BeWo is a choriocarcinoma cell line that generates an extracellular matrix (ECM) rich in laminin and is a useful model for human trophoblast. Immunofluorescence with monoclonal antibodies demonstrates that BeWo ECM contains laminin subunits \u03b21 and \u03b31. Immunoprecipitation from conditioned medium shows that the cells secrete two distinct laminin trimers both containing \u03b21 and \u03b31 but with \u03b1 subunits of approx. 400 and 450\u00a0kDa. The culture medium also contains a species thought to be \u03b21\u03b31 dimer. Immunoprecipitation with monoclonal antibody 4C7, previously thought to recognize the \u03b11 subunit, isolates complexes containing only the smaller \u03b1 subunit. A second complex containing the larger \u03b1 subunit along with \u03b21, \u03b31 and a 150\u00a0kDa polypeptide is precipitated from 4C7-depleted medium with an anti-(laminin 1) polyclonal antibody. Peptide sequencing demonstrates that the 4C7-reactive species is \u03b15, which is present as two similarly sized polypeptides. mRNA species encoding laminin subunits \u03b11, \u03b15, \u03b21, \u03b22 and \u03b31 are all present in the cells. These results demonstrate the secretion of a novel laminin isoform, laminin 10, the subunit composition of which is \u03b15\u03b21\u03b31. Laminin 1 is also produced. No evidence for the secretion of \u03b22-containing laminin isoforms could be derived despite the presence of \u03b22 mRNA. Analysis with reverse transcriptase-mediated PCR also showed the presence of laminin \u03b15 in first-trimester placenta and decidua.<\/jats:p>","DOI":"10.1042\/bj3320491","type":"journal-article","created":{"date-parts":[[2015,8,10]],"date-time":"2015-08-10T22:02:02Z","timestamp":1439244122000},"page":"491-498","update-policy":"https:\/\/doi.org\/10.1042\/crossmark_policy","source":"Crossref","is-referenced-by-count":34,"title":["BeWo choriocarcinoma cells produce laminin 10"],"prefix":"10.1042","volume":"332","author":[{"given":"Heather J.","family":"CHURCH","sequence":"first","affiliation":[{"name":"Department of Obstetrics and Gynaecology and School of Biological Sciences, Research Floor, St Mary's Hospital, Manchester M13 0JH, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John D.","family":"APLIN","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynaecology and School of Biological Sciences, Research Floor, St Mary's Hospital, Manchester M13 0JH, U.K."}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"288","container-title":["Biochemical Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/332\/2\/491\/728746\/bj3320491.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/portlandpress.com\/biochemj\/article-pdf\/332\/2\/491\/728746\/bj3320491.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,11,24]],"date-time":"2021-11-24T01:08:05Z","timestamp":1637716085000},"score":1,"resource":{"primary":{"URL":"https:\/\/portlandpress.com\/biochemj\/article\/332\/2\/491\/38213\/BeWo-choriocarcinoma-cells-produce-laminin-10"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1998,6,1]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1998,6,1]]}},"URL":"https:\/\/doi.org\/10.1042\/bj3320491","relation":{},"ISSN":["0264-6021","1470-8728"],"issn-type":[{"value":"0264-6021","type":"print"},{"value":"1470-8728","type":"electronic"}],"subject":[],"published":{"date-parts":[[1998,6,1]]}}}